Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 177611..178133 Replicon chromosome
Accession NZ_CP059327
Organism Mannheimia haemolytica strain 0729

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1D03_RS00915 Protein ID WP_006249269.1
Coordinates 177611..177907 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag H1D03_RS00920 Protein ID WP_006250574.1
Coordinates 177891..178133 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D03_RS00900 (H1D03_00905) 172664..173989 + 1326 WP_240028742.1 anti-phage deoxyguanosine triphosphatase -
H1D03_RS00905 (H1D03_00910) 174052..174411 + 360 WP_240018399.1 hypothetical protein -
H1D03_RS00910 (H1D03_00915) 174572..177484 + 2913 WP_240028743.1 RNA polymerase-associated protein RapA -
H1D03_RS00915 (H1D03_00920) 177611..177907 + 297 WP_006249269.1 BrnT family toxin Toxin
H1D03_RS00920 (H1D03_00925) 177891..178133 + 243 WP_006250574.1 hypothetical protein Antitoxin
H1D03_RS00925 (H1D03_00930) 178145..178837 + 693 WP_240028744.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1D03_RS00930 (H1D03_00935) 178762..179366 - 605 Protein_186 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1D03_RS00935 (H1D03_00940) 179426..180268 + 843 WP_240028745.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1D03_RS00940 (H1D03_00945) 180390..181031 + 642 WP_006250576.1 orotate phosphoribosyltransferase -
H1D03_RS00945 (H1D03_00950) 181204..182283 + 1080 WP_240018396.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168672 WP_006249269.1 NZ_CP059327:177611-177907 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168672 NZ_CP076216:2312058-2312795 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT168672 WP_006250574.1 NZ_CP059327:177891-178133 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168672 NZ_CP076216:2312908-2313033 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References