Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1806478..1807000 Replicon chromosome
Accession NZ_CP059326
Organism Mannheimia haemolytica strain 0745

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1D04_RS09220 Protein ID WP_006249269.1
Coordinates 1806478..1806774 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag H1D04_RS09225 Protein ID WP_006250574.1
Coordinates 1806758..1807000 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D04_RS09205 (H1D04_09130) 1801530..1802855 + 1326 WP_020831251.1 anti-phage deoxyguanosine triphosphatase -
H1D04_RS09210 (H1D04_09135) 1802918..1803277 + 360 WP_240018399.1 hypothetical protein -
H1D04_RS09215 (H1D04_09140) 1803438..1806350 + 2913 WP_240028743.1 RNA polymerase-associated protein RapA -
H1D04_RS09220 (H1D04_09145) 1806478..1806774 + 297 WP_006249269.1 BrnT family toxin Toxin
H1D04_RS09225 (H1D04_09150) 1806758..1807000 + 243 WP_006250574.1 hypothetical protein Antitoxin
H1D04_RS09230 (H1D04_09155) 1807012..1807683 + 672 WP_275686348.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1D04_RS09235 (H1D04_09160) 1807661..1808235 - 575 Protein_1767 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1D04_RS09240 (H1D04_09165) 1808295..1809137 + 843 Protein_1768 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1D04_RS09245 (H1D04_09170) 1809259..1809900 + 642 WP_275686349.1 orotate phosphoribosyltransferase -
H1D04_RS09250 (H1D04_09175) 1810073..1811155 + 1083 WP_275686350.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168671 WP_006249269.1 NZ_CP059326:1806478-1806774 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168671 NZ_CP076216:241242-241592 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT168671 WP_006250574.1 NZ_CP059326:1806758-1807000 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168671 NZ_CP076216:240950-241237 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References