Detailed information of TA system TA168670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1577888..1578530
• Replicon: chromosome
• Accession: NZ_CP059326
• Organism: Mannheimia haemolytica strain 0745

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H1D04_RS08035
• Protein ID: WP_020831140.1
• Coordinates: 1577888..1578139 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H1D04_RS08040
• Protein ID: WP_240030426.1
• Coordinates: 1578255..1578530 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D04_RS08015	1573029..1575140	+	2112	WP_275686285.1	restriction endonuclease subunit S	-
H1D04_RS08020 (H1D04_07970)	1575143..1575778	+	636	WP_240027581.1	hypothetical protein	-
H1D04_RS08025 (H1D04_07975)	1576009..1576742	-	734	Protein_1526	ABC transporter ATP-binding protein	-
H1D04_RS08030 (H1D04_07980)	1576739..1577707	-	969	WP_240027582.1	iron ABC transporter permease	-
H1D04_RS08035 (H1D04_07985)	1577888..1578139	+	252	WP_020831140.1	hypothetical protein	Toxin
H1D04_RS08040 (H1D04_07990)	1578255..1578530	+	276	WP_240030426.1	putative addiction module antidote protein	Antitoxin
H1D04_RS08045	1578520..1579275	-	756	WP_240028353.1	helical backbone metal receptor	-
H1D04_RS08050	1580092..1580406	-	315	Protein_1531	TonB-dependent receptor plug domain-containing protein	-
H1D04_RS08055 (H1D04_08000)	1580454..1581236	+	783	WP_275686286.1	methylenetetrahydrofolate reductase	-
H1D04_RS08060 (H1D04_08005)	1581285..1582445	-	1161	WP_275686287.1	hypothetical protein	-
H1D04_RS08065 (H1D04_08010)	1582505..1583521	-	1017	WP_020831141.1	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-81)

Antitoxin

• Uncharacterized protein HI1420 (5-88)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9533.89 Da
• Isoelectric Point: 8.3463

>T168670 WP_020831140.1 NZ_CP059326:1577888-1578139 [Mannheimia haemolytica]
MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYIAEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGYKG
TPS

Nucleotide

• Length: 252 bp

>T168670 NZ_CP076213:2293756-2294493 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10025.46 Da
• Isoelectric Point: 4.6283

>AT168670 WP_240030426.1 NZ_CP059326:1578255-1578530 [Mannheimia haemolytica]
MGITEFDAAEYLTNDELIALYLAETLKDGTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIIS
ALNLELSVVKA

Nucleotide

• Length: 276 bp

>AT168670 NZ_CP076213:2294606-2294731 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA