Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 753166..753755 Replicon chromosome
Accession NZ_CP059326
Organism Mannheimia haemolytica strain 0745

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag H1D04_RS03965 Protein ID WP_006248516.1
Coordinates 753423..753755 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag H1D04_RS03960 Protein ID WP_006248517.1
Coordinates 753166..753426 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D04_RS03935 (H1D04_03905) 748340..749485 - 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -
H1D04_RS03940 (H1D04_03910) 750098..750748 + 651 WP_275686756.1 RNA pyrophosphohydrolase -
H1D04_RS03945 (H1D04_03915) 750745..751536 + 792 WP_275686757.1 sulfite exporter TauE/SafE family protein -
H1D04_RS03950 (H1D04_03920) 751544..752341 + 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
H1D04_RS03955 (H1D04_03925) 752428..753096 + 669 WP_240020300.1 phosphoglycolate phosphatase -
H1D04_RS03960 (H1D04_03930) 753166..753426 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1D04_RS03965 (H1D04_03935) 753423..753755 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H1D04_RS03970 (H1D04_03940) 753881..754912 + 1032 WP_240020301.1 tryptophan--tRNA ligase -
H1D04_RS03975 (H1D04_03945) 754975..755730 - 756 WP_240028273.1 M48 family metallopeptidase -
H1D04_RS03980 (H1D04_03950) 755786..756313 - 528 WP_006248512.1 inorganic diphosphatase -
H1D04_RS04000 (H1D04_03970) 756789..757928 + 1140 WP_275686758.1 endolytic transglycosylase MltG -
H1D04_RS04005 (H1D04_03975) 757939..758559 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T168667 WP_006248516.1 NZ_CP059326:753423-753755 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T168667 NZ_CP076210:241049-241399 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT168667 WP_006248517.1 NZ_CP059326:753166-753426 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT168667 NZ_CP076210:240757-241044 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References