Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 557654..558176 Replicon chromosome
Accession NZ_CP059326
Organism Mannheimia haemolytica strain 0745

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag H1D04_RS02815 Protein ID WP_006250322.1
Coordinates 557654..557923 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag H1D04_RS02820 Protein ID WP_006250323.1
Coordinates 557910..558176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D04_RS02795 (H1D04_02770) 552977..553410 - 434 Protein_539 D-aminoacyl-tRNA deacylase -
H1D04_RS02800 (H1D04_02775) 553385..554230 - 846 WP_275686705.1 virulence factor BrkB family protein -
H1D04_RS02805 (H1D04_02780) 554232..555470 - 1239 WP_275686706.1 glutamate-5-semialdehyde dehydrogenase -
H1D04_RS02810 (H1D04_02785) 555752..557635 + 1884 WP_240028202.1 DNA mismatch repair endonuclease MutL -
H1D04_RS02815 (H1D04_02790) 557654..557923 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
H1D04_RS02820 (H1D04_02795) 557910..558176 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
H1D04_RS02825 (H1D04_02800) 558621..559574 + 954 WP_240028203.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
H1D04_RS02830 (H1D04_02805) 559643..559915 + 273 WP_006250326.1 RNA chaperone Hfq -
H1D04_RS02835 (H1D04_02810) 559935..561323 + 1389 WP_275686707.1 GTPase HflX -
H1D04_RS02840 (H1D04_02815) 561657..562697 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 561657..562697 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T168665 WP_006250322.1 NZ_CP059326:c557923-557654 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T168665 NZ_CP076207:241540-241890 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT168665 WP_006250323.1 NZ_CP059326:c558176-557910 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT168665 NZ_CP076207:241248-241535 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References