Detailed information of TA system TA168659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1635962..1636379
• Replicon: chromosome
• Accession: NZ_CP059325
• Organism: Mannheimia haemolytica strain 0746

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H1D05_RS08075
• Protein ID: WP_240019294.1
• Coordinates: 1635962..1636153 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A249A190
• Locus tag: H1D05_RS08080
• Protein ID: WP_006249270.1
• Coordinates: 1636137..1636379 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D05_RS08065 (H1D05_08060)	1632298..1632657	+	360	WP_240018399.1	hypothetical protein	-
H1D05_RS08070 (H1D05_08065)	1632818..1635730	+	2913	WP_240019293.1	RNA polymerase-associated protein RapA	-
H1D05_RS08075	1635962..1636153	+	192	WP_240019294.1	BrnT family toxin	Toxin
H1D05_RS08080 (H1D05_08075)	1636137..1636379	+	243	WP_006249270.1	CopG family antitoxin	Antitoxin
H1D05_RS08085 (H1D05_08080)	1636391..1637059	+	669	WP_240019295.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
H1D05_RS08090 (H1D05_08085)	1637037..1637611	-	575	Protein_1555	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
H1D05_RS08095 (H1D05_08090)	1637671..1638513	+	843	WP_240019296.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
H1D05_RS08100 (H1D05_08095)	1638635..1639276	+	642	WP_006249274.1	orotate phosphoribosyltransferase	-
H1D05_RS08105 (H1D05_08100)	1639449..1640540	+	1092	WP_240019297.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (3-55)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7906.21 Da
• Isoelectric Point: 9.3589

>T168659 WP_240019294.1 NZ_CP059325:1635962-1636153 [Mannheimia haemolytica]
MLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH

Nucleotide

• Length: 192 bp

>T168659 NZ_CP076198:241086-241436 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9346.79 Da
• Isoelectric Point: 7.1665

>AT168659 WP_006249270.1 NZ_CP059325:1636137-1636379 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Nucleotide

• Length: 243 bp

>AT168659 NZ_CP076198:240794-241081 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A249A190