Detailed information of TA system TA168658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 1344198..1344848
• Replicon: chromosome
• Accession: NZ_CP059325
• Organism: Mannheimia haemolytica strain 0746

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1D05_RS06610
• Protein ID: WP_240020768.1
• Coordinates: 1344459..1344848 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A547EDM1
• Locus tag: H1D05_RS06605
• Protein ID: WP_006249412.1
• Coordinates: 1344198..1344458 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D05_RS06590 (H1D05_06585)	1339883..1343419	+	3537	WP_240020766.1	transcription-repair coupling factor	-
H1D05_RS06595 (H1D05_06590)	1343582..1343869	+	288	WP_006251764.1	BrnT family toxin	-
H1D05_RS06600 (H1D05_06595)	1343870..1344082	+	213	WP_006251765.1	BrnA antitoxin family protein	-
H1D05_RS06605 (H1D05_06600)	1344198..1344458	+	261	WP_006249412.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H1D05_RS06610 (H1D05_06605)	1344459..1344848	+	390	WP_240020768.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1D05_RS06615 (H1D05_06610)	1344868..1346112	+	1245	WP_240020770.1	peptidase T	-
H1D05_RS06645 (H1D05_06640)	1346854..1347642	+	789	WP_240020772.1	GDP-L-fucose synthase	-
H1D05_RS06650 (H1D05_06645)	1347716..1348576	+	861	WP_240020774.1	metal ABC transporter permease	-
H1D05_RS06655 (H1D05_06650)	1348569..1349378	+	810	Protein_1266	metal ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14470.74 Da
• Isoelectric Point: 7.1048

>T168658 WP_240020768.1 NZ_CP059325:1344459-1344848 [Mannheimia haemolytica]
MYMLDTNTVSFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLR
AEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Nucleotide

• Length: 390 bp

>T168658 NZ_CP076195:2316476-2317213 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10245.54 Da
• Isoelectric Point: 4.8951

>AT168658 WP_006249412.1 NZ_CP059325:1344198-1344458 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Nucleotide

• Length: 261 bp

>AT168658 NZ_CP076195:2317326-2317451 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A547EDM1