Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 565731..566320 Replicon chromosome
Accession NZ_CP059325
Organism Mannheimia haemolytica strain 0746

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag H1D05_RS02790 Protein ID WP_006248516.1
Coordinates 565988..566320 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag H1D05_RS02785 Protein ID WP_006248517.1
Coordinates 565731..565991 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D05_RS02760 (H1D05_02750) 560909..562054 - 1146 WP_240020298.1 FMN-dependent L-lactate dehydrogenase LldD -
H1D05_RS02765 (H1D05_02755) 562666..563316 + 651 WP_240020299.1 RNA pyrophosphohydrolase -
H1D05_RS02770 (H1D05_02760) 563313..564101 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
H1D05_RS02775 (H1D05_02765) 564109..564906 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
H1D05_RS02780 (H1D05_02770) 564993..565661 + 669 WP_240020300.1 phosphoglycolate phosphatase -
H1D05_RS02785 (H1D05_02775) 565731..565991 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1D05_RS02790 (H1D05_02780) 565988..566320 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H1D05_RS02795 (H1D05_02785) 566447..567478 + 1032 WP_240020301.1 tryptophan--tRNA ligase -
H1D05_RS02800 (H1D05_02790) 567541..568296 - 756 WP_240020302.1 M48 family metallopeptidase -
H1D05_RS02805 (H1D05_02795) 568352..568879 - 528 WP_006248512.1 inorganic diphosphatase -
H1D05_RS02825 (H1D05_02815) 569355..570491 + 1137 WP_236610598.1 endolytic transglycosylase MltG -
H1D05_RS02830 (H1D05_02820) 570502..571125 + 624 WP_240020814.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T168656 WP_006248516.1 NZ_CP059325:565988-566320 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T168656 NZ_CP076192:2317057-2317794 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT168656 WP_006248517.1 NZ_CP059325:565731-565991 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT168656 NZ_CP076192:2317907-2318032 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References