Detailed information of TA system TA168655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 522418..522940
• Replicon: chromosome
• Accession: NZ_CP059325
• Organism: Mannheimia haemolytica strain 0746

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H1D05_RS02585
• Protein ID: WP_240020276.1
• Coordinates: 522418..522687 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1D05_RS02590
• Protein ID: WP_006250323.1
• Coordinates: 522674..522940 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D05_RS02565 (H1D05_02555)	517734..518168	-	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-
H1D05_RS02570 (H1D05_02560)	518143..518988	-	846	WP_006250319.1	virulence factor BrkB family protein	-
H1D05_RS02575 (H1D05_02565)	518990..520231	-	1242	WP_240020274.1	glutamate-5-semialdehyde dehydrogenase	-
H1D05_RS02580 (H1D05_02570)	520513..522399	+	1887	WP_240020275.1	DNA mismatch repair endonuclease MutL	-
H1D05_RS02585 (H1D05_02575)	522418..522687	-	270	WP_240020276.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H1D05_RS02590 (H1D05_02580)	522674..522940	-	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1D05_RS02595 (H1D05_02585)	523380..524333	+	954	WP_240020277.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1D05_RS02600 (H1D05_02590)	524402..524674	+	273	WP_006250326.1	RNA chaperone Hfq	-
H1D05_RS02605 (H1D05_02595)	524694..526082	+	1389	WP_240020278.1	GTPase HflX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10319.82 Da
• Isoelectric Point: 6.0718

>T168655 WP_240020276.1 NZ_CP059325:c522687-522418 [Mannheimia haemolytica]
MREIEYSNAFKRDYKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRI
GSHSHLFG

Nucleotide

• Length: 270 bp

>T168655 NZ_CP076192:241541-241891 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168655 WP_006250323.1 NZ_CP059325:c522940-522674 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168655 NZ_CP076192:241249-241536 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54