Detailed information of TA system TA168652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 1584070..1584592
• Replicon: chromosome
• Accession: NZ_CP059324
• Organism: Mannheimia haemolytica strain 0760

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H1D06_RS07970
• Protein ID: WP_240017651.1
• Coordinates: 1584323..1584592 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1D06_RS07965
• Protein ID: WP_006250323.1
• Coordinates: 1584070..1584336 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D06_RS07945 (H1D06_07960)	1579549..1580589	-	1041	WP_020824044.1	IS481 family transposase	-
H1D06_RS07950 (H1D06_07965)	1580923..1582311	-	1389	WP_240017649.1	GTPase HflX	-
H1D06_RS07955 (H1D06_07970)	1582331..1582603	-	273	WP_006250326.1	RNA chaperone Hfq	-
H1D06_RS07960 (H1D06_07975)	1582672..1583625	-	954	WP_240017650.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1D06_RS07965 (H1D06_07980)	1584070..1584336	+	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1D06_RS07970 (H1D06_07985)	1584323..1584592	+	270	WP_240017651.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H1D06_RS07975 (H1D06_07990)	1584611..1586494	-	1884	WP_240017652.1	DNA mismatch repair endonuclease MutL	-
H1D06_RS07980 (H1D06_07995)	1586776..1588014	+	1239	WP_240017653.1	glutamate-5-semialdehyde dehydrogenase	-
H1D06_RS07985 (H1D06_08000)	1588016..1588861	+	846	WP_006251985.1	virulence factor BrkB family protein	-
H1D06_RS07990 (H1D06_08005)	1588836..1589270	+	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1579549..1580589	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10447.99 Da
• Isoelectric Point: 6.4732

>T168652 WP_240017651.1 NZ_CP059324:1584323-1584592 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T168652 NZ_CP076187:2302775-2303512 [Bacillus anthracis str. BF1]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168652 WP_006250323.1 NZ_CP059324:1584070-1584336 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168652 NZ_CP076187:2303625-2303750 [Bacillus anthracis str. BF1]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54