Detailed information of TA system TA168651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1438164..1438813
• Replicon: chromosome
• Accession: NZ_CP059324
• Organism: Mannheimia haemolytica strain 0760

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: H1D06_RS07080
• Protein ID: WP_006251221.1
• Coordinates: 1438164..1438340 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: H1D06_RS07085
• Protein ID: WP_006251222.1
• Coordinates: 1438397..1438813 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D06_RS07055 (H1D06_07050)	1433474..1435969	-	2496	WP_240017572.1	phage tail length tape measure family protein	-
H1D06_RS07060 (H1D06_07055)	1436056..1436451	-	396	WP_006251217.1	hypothetical protein	-
H1D06_RS07065 (H1D06_07060)	1436518..1436979	-	462	WP_240017573.1	hypothetical protein	-
H1D06_RS07070 (H1D06_07065)	1437148..1437381	+	234	WP_020824316.1	hypothetical protein	-
H1D06_RS07075 (H1D06_07070)	1437396..1438061	-	666	WP_240017574.1	DUF6246 family protein	-
H1D06_RS07080 (H1D06_07075)	1438164..1438340	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
H1D06_RS07085 (H1D06_07080)	1438397..1438813	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H1D06_RS07090 (H1D06_07085)	1438853..1439335	-	483	WP_006251223.1	phage tail tube protein	-
H1D06_RS07095 (H1D06_07090)	1439339..1439719	-	381	WP_240017575.1	hypothetical protein	-
H1D06_RS07100 (H1D06_07095)	1439716..1440084	-	369	WP_006251225.1	hypothetical protein	-
H1D06_RS07105 (H1D06_07100)	1440086..1440430	-	345	WP_006251226.1	hypothetical protein	-
H1D06_RS07110 (H1D06_07110)	1440766..1441044	-	279	WP_240017576.1	hypothetical protein	-
H1D06_RS07115 (H1D06_07115)	1441056..1442042	-	987	WP_240017577.1	encapsulin	-
H1D06_RS07120 (H1D06_07120)	1442057..1442491	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1424490..1475275	50785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T168651 WP_006251221.1 NZ_CP059324:1438164-1438340 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T168651 NZ_CP076187:241397-241747 [Bacillus anthracis str. BF1]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT168651 WP_006251222.1 NZ_CP059324:1438397-1438813 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT168651 NZ_CP076187:241105-241392 [Bacillus anthracis str. BF1]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6