Detailed information of TA system TA168650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1388476..1389068
• Replicon: chromosome
• Accession: NZ_CP059324
• Organism: Mannheimia haemolytica strain 0760

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H1D06_RS06830
• Protein ID: WP_240017548.1
• Coordinates: 1388476..1388811 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A248ZX26
• Locus tag: H1D06_RS06835
• Protein ID: WP_006248517.1
• Coordinates: 1388808..1389068 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D06_RS06790 (H1D06_06790)	1383671..1384291	-	621	WP_240018871.1	dTMP kinase	-
H1D06_RS06795 (H1D06_06795)	1384302..1385441	-	1140	WP_240017546.1	endolytic transglycosylase MltG	-
H1D06_RS06815 (H1D06_06815)	1385917..1386444	+	528	WP_006248512.1	inorganic diphosphatase	-
H1D06_RS06820 (H1D06_06820)	1386500..1387255	+	756	WP_006248513.1	M48 family metallopeptidase	-
H1D06_RS06825 (H1D06_06825)	1387319..1388350	-	1032	WP_240017547.1	tryptophan--tRNA ligase	-
H1D06_RS06830 (H1D06_06830)	1388476..1388811	-	336	WP_240017548.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H1D06_RS06835 (H1D06_06835)	1388808..1389068	-	261	WP_006248517.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H1D06_RS06840 (H1D06_06840)	1389138..1389806	-	669	WP_006248518.1	phosphoglycolate phosphatase	-
H1D06_RS06845 (H1D06_06845)	1389893..1390690	-	798	WP_006251103.1	prolipoprotein diacylglyceryl transferase	-
H1D06_RS06850 (H1D06_06850)	1390698..1391486	-	789	WP_240017549.1	sulfite exporter TauE/SafE family protein	-
H1D06_RS06855 (H1D06_06855)	1391488..1392132	-	645	WP_240017550.1	RNA pyrophosphohydrolase	-
H1D06_RS06860 (H1D06_06860)	1392744..1393889	+	1146	WP_006251102.1	FMN-dependent L-lactate dehydrogenase LldD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-110)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12121.09 Da
• Isoelectric Point: 6.4597

>T168650 WP_240017548.1 NZ_CP059324:c1388811-1388476 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSLATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRT
VDIAQRHGKFIEALPESLLDEIMARVSVIFE

Nucleotide

• Length: 336 bp

>T168650 NZ_CP076184:2311628-2312365 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9418.88 Da
• Isoelectric Point: 4.7228

>AT168650 WP_006248517.1 NZ_CP059324:c1389068-1388808 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Nucleotide

• Length: 261 bp

>AT168650 NZ_CP076184:2312478-2312603 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A248ZX26