Detailed information of TA system TA168648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 648424..649074
• Replicon: chromosome
• Accession: NZ_CP059324
• Organism: Mannheimia haemolytica strain 0760

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1D06_RS03190
• Protein ID: WP_240018543.1
• Coordinates: 648424..648813 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1D2Q6P1
• Locus tag: H1D06_RS03195
• Protein ID: WP_006251766.1
• Coordinates: 648814..649074 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D06_RS03140 (H1D06_03125)	643655..644463	-	809	Protein_620	metal ABC transporter permease	-
H1D06_RS03145 (H1D06_03130)	644456..645316	-	861	WP_240018542.1	metal ABC transporter permease	-
H1D06_RS03185 (H1D06_03175)	647166..648404	-	1239	WP_006251767.1	peptidase T	-
H1D06_RS03190 (H1D06_03180)	648424..648813	-	390	WP_240018543.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1D06_RS03195 (H1D06_03185)	648814..649074	-	261	WP_006251766.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H1D06_RS03200 (H1D06_03190)	649190..649402	-	213	WP_006251765.1	BrnA antitoxin family protein	-
H1D06_RS03205	649403..649492	-	90	WP_240018853.1	BrnT family toxin	-
H1D06_RS03210	649558..649689	-	132	Protein_627	BrnT family toxin	-
H1D06_RS03215 (H1D06_03200)	649851..653384	-	3534	WP_240018544.1	transcription-repair coupling factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14575.87 Da
• Isoelectric Point: 7.7391

>T168648 WP_240018543.1 NZ_CP059324:c648813-648424 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Nucleotide

• Length: 390 bp

>T168648 NZ_CP076181:2317066-2317803 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10245.50 Da
• Isoelectric Point: 4.6909

>AT168648 WP_006251766.1 NZ_CP059324:c649074-648814 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Nucleotide

• Length: 261 bp

>AT168648 NZ_CP076181:2317916-2318041 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1D2Q6P1