Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 335365..335887 Replicon chromosome
Accession NZ_CP059324
Organism Mannheimia haemolytica strain 0760

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1D06_RS01635 Protein ID WP_006249269.1
Coordinates 335591..335887 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag H1D06_RS01630 Protein ID WP_006250574.1
Coordinates 335365..335607 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D06_RS01605 (H1D06_01590) 331212..332291 - 1080 WP_240018396.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
H1D06_RS01610 (H1D06_01595) 332464..333105 - 642 WP_006250576.1 orotate phosphoribosyltransferase -
H1D06_RS01615 (H1D06_01600) 333228..334073 - 846 WP_240018397.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1D06_RS01620 (H1D06_01605) 334133..334707 + 575 Protein_315 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1D06_RS01625 (H1D06_01610) 334685..335353 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1D06_RS01630 (H1D06_01615) 335365..335607 - 243 WP_006250574.1 hypothetical protein Antitoxin
H1D06_RS01635 (H1D06_01620) 335591..335887 - 297 WP_006249269.1 BrnT family toxin Toxin
H1D06_RS01640 (H1D06_01625) 336014..338926 - 2913 WP_240018398.1 RNA polymerase-associated protein RapA -
H1D06_RS01645 (H1D06_01630) 339090..339449 - 360 WP_240018399.1 hypothetical protein -
H1D06_RS01650 (H1D06_01635) 339512..340837 - 1326 WP_240018400.1 anti-phage deoxyguanosine triphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168647 WP_006249269.1 NZ_CP059324:c335887-335591 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168647 NZ_CP076181:241541-241891 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT168647 WP_006250574.1 NZ_CP059324:c335607-335365 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168647 NZ_CP076181:241249-241536 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References