Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2426769..2427358 Replicon chromosome
Accession NZ_CP059323
Organism Mannheimia haemolytica strain 0768

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag H1D07_RS11845 Protein ID WP_006248516.1
Coordinates 2426769..2427101 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag H1D07_RS11850 Protein ID WP_006248517.1
Coordinates 2427098..2427358 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D07_RS11805 (H1D07_11855) 2421964..2422584 - 621 WP_240018871.1 dTMP kinase -
H1D07_RS11810 (H1D07_11860) 2422595..2423734 - 1140 WP_240024938.1 endolytic transglycosylase MltG -
H1D07_RS11830 (H1D07_11880) 2424210..2424737 + 528 WP_006248512.1 inorganic diphosphatase -
H1D07_RS11835 (H1D07_11885) 2424793..2425548 + 756 WP_006248513.1 M48 family metallopeptidase -
H1D07_RS11840 (H1D07_11890) 2425611..2426642 - 1032 WP_240017547.1 tryptophan--tRNA ligase -
H1D07_RS11845 (H1D07_11895) 2426769..2427101 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H1D07_RS11850 (H1D07_11900) 2427098..2427358 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1D07_RS11855 (H1D07_11905) 2427428..2428099 - 672 WP_240024939.1 phosphoglycolate phosphatase -
H1D07_RS11860 (H1D07_11910) 2428186..2428983 - 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
H1D07_RS11865 (H1D07_11915) 2428991..2429785 - 795 WP_240026892.1 sulfite exporter TauE/SafE family protein -
H1D07_RS11870 (H1D07_11920) 2429787..2430431 - 645 WP_240017550.1 RNA pyrophosphohydrolase -
H1D07_RS11875 (H1D07_11925) 2431043..2432188 + 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T168645 WP_006248516.1 NZ_CP059323:c2427101-2426769 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T168645 NZ_CP076178:241542-241892 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT168645 WP_006248517.1 NZ_CP059323:c2427358-2427098 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT168645 NZ_CP076178:241250-241537 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References