Detailed information of TA system TA168643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 1686834..1687484
• Replicon: chromosome
• Accession: NZ_CP059323
• Organism: Mannheimia haemolytica strain 0768

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1D07_RS08195
• Protein ID: WP_240018543.1
• Coordinates: 1686834..1687223 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1D2Q6P1
• Locus tag: H1D07_RS08200
• Protein ID: WP_006251766.1
• Coordinates: 1687224..1687484 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D07_RS08145 (H1D07_08190)	1682069..1682877	-	809	Protein_1605	metal ABC transporter permease	-
H1D07_RS08150 (H1D07_08195)	1682870..1683730	-	861	WP_240018542.1	metal ABC transporter permease	-
H1D07_RS08190 (H1D07_08240)	1685576..1686814	-	1239	WP_006251767.1	peptidase T	-
H1D07_RS08195 (H1D07_08245)	1686834..1687223	-	390	WP_240018543.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1D07_RS08200 (H1D07_08250)	1687224..1687484	-	261	WP_006251766.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H1D07_RS08205 (H1D07_08255)	1687600..1687812	-	213	WP_006251765.1	BrnA antitoxin family protein	-
H1D07_RS08210	1687813..1687902	-	90	WP_240018853.1	BrnT family toxin	-
H1D07_RS08215	1687968..1688099	-	132	Protein_1612	BrnT family toxin	-
H1D07_RS08220 (H1D07_08265)	1688261..1691800	-	3540	WP_240026709.1	transcription-repair coupling factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14575.87 Da
• Isoelectric Point: 7.7391

>T168643 WP_240018543.1 NZ_CP059323:c1687223-1686834 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Nucleotide

• Length: 390 bp

>T168643 NZ_CP076176:241542-241892 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10245.50 Da
• Isoelectric Point: 4.6909

>AT168643 WP_006251766.1 NZ_CP059323:c1687484-1687224 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Nucleotide

• Length: 261 bp

>AT168643 NZ_CP076176:241250-241537 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D2Q6P1