Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1373795..1374317 Replicon chromosome
Accession NZ_CP059323
Organism Mannheimia haemolytica strain 0768

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1D07_RS06645 Protein ID WP_006249269.1
Coordinates 1374021..1374317 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag H1D07_RS06640 Protein ID WP_006250574.1
Coordinates 1373795..1374037 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D07_RS06615 (H1D07_06655) 1369643..1370722 - 1080 WP_240026616.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
H1D07_RS06620 (H1D07_06660) 1370895..1371536 - 642 WP_006250576.1 orotate phosphoribosyltransferase -
H1D07_RS06625 (H1D07_06665) 1371658..1372503 - 846 WP_240020975.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1D07_RS06630 (H1D07_06670) 1372563..1373137 + 575 Protein_1302 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1D07_RS06635 (H1D07_06675) 1373115..1373783 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1D07_RS06640 (H1D07_06680) 1373795..1374037 - 243 WP_006250574.1 hypothetical protein Antitoxin
H1D07_RS06645 (H1D07_06685) 1374021..1374317 - 297 WP_006249269.1 BrnT family toxin Toxin
H1D07_RS06650 (H1D07_06690) 1374444..1377356 - 2913 WP_240026617.1 RNA polymerase-associated protein RapA -
H1D07_RS06655 (H1D07_06695) 1377517..1377876 - 360 WP_240018399.1 hypothetical protein -
H1D07_RS06660 (H1D07_06700) 1377939..1379264 - 1326 WP_240026618.1 anti-phage deoxyguanosine triphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168642 WP_006249269.1 NZ_CP059323:c1374317-1374021 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168642 NZ_CP076173:2311510-2312247 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT168642 WP_006250574.1 NZ_CP059323:c1374037-1373795 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168642 NZ_CP076173:2312360-2312485 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References