Detailed information of TA system TA168640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 32036..32558
• Replicon: chromosome
• Accession: NZ_CP059323
• Organism: Mannheimia haemolytica strain 0768

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A248ZX63
• Locus tag: H1D07_RS00160
• Protein ID: WP_006250322.1
• Coordinates: 32289..32558 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1D07_RS00155
• Protein ID: WP_006250323.1
• Coordinates: 32036..32302 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D07_RS00135 (H1D07_00130)	27517..28557	-	1041	WP_020824044.1	IS481 family transposase	-
H1D07_RS00140 (H1D07_00135)	28892..30277	-	1386	WP_020824252.1	GTPase HflX	-
H1D07_RS00145 (H1D07_00140)	30297..30569	-	273	WP_006250326.1	RNA chaperone Hfq	-
H1D07_RS00150 (H1D07_00145)	30639..31592	-	954	WP_240021449.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1D07_RS00155 (H1D07_00150)	32036..32302	+	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1D07_RS00160 (H1D07_00155)	32289..32558	+	270	WP_006250322.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
H1D07_RS00165 (H1D07_00160)	32577..34460	-	1884	WP_240017652.1	DNA mismatch repair endonuclease MutL	-
H1D07_RS00170 (H1D07_00165)	34741..35982	+	1242	WP_240026944.1	glutamate-5-semialdehyde dehydrogenase	-
H1D07_RS00175 (H1D07_00170)	35984..36829	+	846	WP_006251985.1	virulence factor BrkB family protein	-
H1D07_RS00180 (H1D07_00175)	36804..37238	+	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	27517..28557	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10447.99 Da
• Isoelectric Point: 6.4732

>T168640 WP_006250322.1 NZ_CP059323:32289-32558 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T168640 NZ_CP076170:2316958-2317695 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168640 WP_006250323.1 NZ_CP059323:32036-32302 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168640 NZ_CP076170:2317808-2317933 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A248ZX63

Antitoxin

• AlphaFold DB: A0A248ZY54