Detailed information of TA system TA168635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 372505..373054
• Replicon: chromosome
• Accession: NZ_CP059319
• Organism: Rhizorhabdus wittichii strain MPO218

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A975HFW0
• Locus tag: HRJ34_RS01770
• Protein ID: WP_029994977.1
• Coordinates: 372505..372840 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A975HEC6
• Locus tag: HRJ34_RS01775
• Protein ID: WP_016747094.1
• Coordinates: 372830..373054 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ34_RS01740 (HRJ34_01745)	368341..369162	-	822	WP_029994971.1	class I SAM-dependent methyltransferase	-
HRJ34_RS01745 (HRJ34_01750)	369419..369724	+	306	WP_208633180.1	type II toxin-antitoxin system HicB family antitoxin	-
HRJ34_RS01750 (HRJ34_01755)	369728..370096	-	369	WP_208633181.1	hypothetical protein	-
HRJ34_RS01755 (HRJ34_01760)	370078..370827	-	750	WP_029549014.1	HesA/MoeB/ThiF family protein	-
HRJ34_RS01760 (HRJ34_01765)	370855..371304	-	450	WP_012051052.1	dUTP diphosphatase	-
HRJ34_RS01765 (HRJ34_01770)	371291..372508	-	1218	WP_208633182.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
HRJ34_RS01770 (HRJ34_01775)	372505..372840	-	336	WP_029994977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HRJ34_RS01775 (HRJ34_01780)	372830..373054	-	225	WP_016747094.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HRJ34_RS01780 (HRJ34_01785)	373087..374614	-	1528	Protein_357	2-polyprenylphenol 6-hydroxylase	-
HRJ34_RS01785 (HRJ34_01790)	374611..375342	-	732	WP_208633183.1	class I SAM-dependent methyltransferase	-
HRJ34_RS01790 (HRJ34_01795)	375562..376374	+	813	WP_029994980.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
HRJ34_RS01795 (HRJ34_01800)	376482..376745	+	264	WP_012051059.1	30S ribosomal protein S20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-108)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12516.41 Da
• Isoelectric Point: 10.7007

>T168635 WP_029994977.1 NZ_CP059319:c372840-372505 [Rhizorhabdus wittichii]
VTFDRFDTVVVPFPFTDRRTTKRRPALMLSDRAFGEATDNALLAMITSAEQSDWPGDTALSDRERAGLPKPCKVRMKLFT
LDHRLIERRIGTLSQADRDAVVRALRTVVGL

Nucleotide

• Length: 336 bp

>T168635 NZ_CP076164:241541-241891 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8147.08 Da
• Isoelectric Point: 4.5238

>AT168635 WP_016747094.1 NZ_CP059319:c373054-372830 [Rhizorhabdus wittichii]
MDVSKLTAKNQVTIPADVRQQLGVKSGDHVAFEVEDGKVIVRKARAIDWAWTATVGSTLSEWSSDADEEAFRDL

Nucleotide

• Length: 225 bp

>AT168635 NZ_CP076164:241249-241536 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA