Detailed information of TA system TA168634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1237177..1238094
• Replicon: chromosome
• Accession: NZ_CP059318
• Organism: Bacillus velezensis strain BIOMA BV10

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HZT45_RS06415
• Protein ID: WP_052827326.1
• Coordinates: 1237348..1238094 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HZT45_RS06410
• Protein ID: WP_003154807.1
• Coordinates: 1237177..1237347 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT45_RS06375	1232401..1234032	+	1632	WP_052827325.1	hypothetical protein	-
HZT45_RS06380	1234045..1234416	+	372	WP_043021266.1	YomQ/XkdW protein, phage-like element PBSX	-
HZT45_RS06385	1234421..1234618	+	198	WP_012117366.1	XkdX family protein	-
HZT45_RS06390	1234675..1235436	+	762	WP_181467532.1	phage portal protein	-
HZT45_RS06395	1235488..1235751	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HZT45_RS06400	1235765..1236028	+	264	WP_003154813.1	phage holin	-
HZT45_RS06405	1236042..1236920	+	879	WP_043021264.1	N-acetylmuramoyl-L-alanine amidase	-
HZT45_RS06410	1237177..1237347	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HZT45_RS06415	1237348..1238094	-	747	WP_052827326.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HZT45_RS06420	1238202..1239200	-	999	WP_007407255.1	inorganic phosphate transporter	-
HZT45_RS06425	1239213..1239830	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HZT45_RS06430	1240116..1241432	-	1317	WP_007407254.1	amino acid permease	-
HZT45_RS06435	1241755..1242705	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29067.53 Da
• Isoelectric Point: 4.7755

>T168634 WP_052827326.1 NZ_CP059318:c1238094-1237348 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDSESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T168634 NZ_CP076163:2316769-2317506 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT168634 WP_003154807.1 NZ_CP059318:c1237347-1237177 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT168634 NZ_CP076163:2317619-2317744 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14