Detailed information of TA system TA168633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 489186..489823
• Replicon: chromosome
• Accession: NZ_CP059318
• Organism: Bacillus velezensis strain BIOMA BV10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HZT45_RS02440
• Protein ID: WP_003156187.1
• Coordinates: 489473..489823 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HZT45_RS02435
• Protein ID: WP_003156188.1
• Coordinates: 489186..489467 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT45_RS02415	485551..486150	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
HZT45_RS02420	486243..486608	+	366	WP_007410229.1	holo-ACP synthase	-
HZT45_RS02425	486773..487780	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
HZT45_RS02430	487897..489066	+	1170	WP_025284272.1	alanine racemase	-
HZT45_RS02435	489186..489467	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HZT45_RS02440	489473..489823	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HZT45_RS02445	489941..490762	+	822	WP_003156182.1	STAS domain-containing protein	-
HZT45_RS02450	490767..491132	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HZT45_RS02455	491135..491536	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HZT45_RS02460	491548..492555	+	1008	WP_052827076.1	PP2C family protein-serine/threonine phosphatase	-
HZT45_RS02465	492619..492948	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HZT45_RS02470	492945..493427	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
HZT45_RS02475	493393..494181	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
HZT45_RS02480	494181..494783	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T168633 WP_003156187.1 NZ_CP059318:489473-489823 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T168633 NZ_CP076163:241542-241892 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT168633 WP_003156188.1 NZ_CP059318:489186-489467 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT168633 NZ_CP076163:241250-241537 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5