Detailed information of TA system TA168618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1468901..1469637
• Replicon: chromosome
• Accession: NZ_CP059317
• Organism: Klebsiella pneumoniae strain Kp46564

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: H1D29_RS08700
• Protein ID: WP_032433360.1
• Coordinates: 1469155..1469637 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H1D29_RS08695
• Protein ID: WP_003026799.1
• Coordinates: 1468901..1469167 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D29_RS08670	1464547..1465686	+	1140	WP_032433368.1	mannitol dehydrogenase	-
H1D29_RS08675	1465715..1466377	+	663	WP_032433366.1	dihydroxyacetone kinase subunit L	-
H1D29_RS08680	1466361..1467365	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
H1D29_RS08685	1467383..1468015	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
H1D29_RS08690	1468025..1468588	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
H1D29_RS08695	1468901..1469167	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H1D29_RS08700	1469155..1469637	+	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
H1D29_RS08705	1469998..1470597	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
H1D29_RS08710	1470810..1471754	-	945	WP_077254249.1	fimbrial protein	-
H1D29_RS08715	1471766..1472344	-	579	WP_032432061.1	type 1 fimbrial protein	-
H1D29_RS08720	1472348..1473088	-	741	WP_050597964.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1436846..1480580	43734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T168618 WP_032433360.1 NZ_CP059317:1469155-1469637 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T168618 NZ_CP076141:2316995-2317732 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT168618 WP_003026799.1 NZ_CP059317:1468901-1469167 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT168618 NZ_CP076141:2317845-2317970 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66