Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5212921..5213546 Replicon chromosome
Accession NZ_CP059312
Organism Klebsiella pneumoniae strain Kp46596

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H1D28_RS28040 Protein ID WP_002882817.1
Coordinates 5212921..5213304 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H1D28_RS28045 Protein ID WP_004150355.1
Coordinates 5213304..5213546 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D28_RS28025 5210287..5211189 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H1D28_RS28030 5211186..5211821 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H1D28_RS28035 5211818..5212747 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H1D28_RS28040 5212921..5213304 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H1D28_RS28045 5213304..5213546 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H1D28_RS28050 5213751..5214668 + 918 WP_021466676.1 alpha/beta hydrolase -
H1D28_RS28055 5214682..5215623 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H1D28_RS28060 5215668..5216105 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H1D28_RS28065 5216102..5216962 - 861 WP_004146232.1 virulence factor BrkB family protein -
H1D28_RS28070 5216956..5217555 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T168612 WP_002882817.1 NZ_CP059312:c5213304-5212921 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T168612 NZ_CP076136:2459766-2460050 [Bradyrhizobium sediminis]
GTGATTGTCAGCTATCGGGACAAGGCCACCGAGCGGTTTGCCTCCGGACGACATGTCAGGGAGTTCTCGGGCTTTGCACG
ACAGGCTGAGATGCGTCTTGATCGGCTGGAGACGGCCGCAAGTCTCGCCGATTTGGCGGCCCTGCCTGGTAACCGACTGG
AAGCGCTCAAAGGCGACCGCGCCGGACAGTTTTCAATCCGCATCAACGATCAATGGCGGATCCGCTTCAGGTGGCCTGAA
GGATCGCCCGGCCCAGTGGACGTGGAAATCGTCGATTATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT168612 WP_004150355.1 NZ_CP059312:c5213546-5213304 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT168612 NZ_CP076136:2460063-2460380 [Bradyrhizobium sediminis]
ATGCCTCGTGCTGCCATCCATCCCGGTGAACATCTTGCCGAAGAATTGCGCGAGCTTGGCATCACGGCTGCCGAGCTTTC
GCGCCAAATCGATGTGCCGGTGAATCGCATTACCGGCATTATTCACGGCCAGCGCGGCATCACGGCCGATACGGCGCTGC
GTCTTGGGCACTGGTTTGGAACGGCACCACAATTCTGGATGAATTTGCAGCAACTCTACGAACTGCGCCTCGCGGAAAAT
GAAATTGGCGCGGAAATCGCGGCACTGCCGCGTCGTGCAGGTCGGTCGACACCACGCAAGCTTGGGAAAACAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References