Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4618903..4619713 Replicon chromosome
Accession NZ_CP059312
Organism Klebsiella pneumoniae strain Kp46596

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag H1D28_RS25265 Protein ID WP_002887280.1
Coordinates 4618903..4619436 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag H1D28_RS25270 Protein ID WP_002887278.1
Coordinates 4619447..4619713 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D28_RS25260 4617735..4618856 + 1122 WP_009309849.1 cupin domain-containing protein -
H1D28_RS25265 4618903..4619436 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
H1D28_RS25270 4619447..4619713 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
H1D28_RS25275 4619816..4621249 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
H1D28_RS25280 4621239..4621922 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
H1D28_RS25285 4622095..4623480 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
H1D28_RS25290 4623498..4623803 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T168609 WP_002887280.1 NZ_CP059312:c4619436-4618903 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T168609 NZ_CP076135:c4794122-4793751 [Bradyrhizobium sediminis]
ATGGTACTTGCCGATACGTCGATCTGGATAGATCATTTTCGTCGTGGGGATTTTCAATTATCCGAGCTGCTTGAGCGTGG
CGATATCGTCATACATCCGTTCATCGTTGGGGAATTGTTACTGGGACACGTGCCCAGGATCGCCGAGATGCTCGATGATT
TGAACGTTCTTCCAAGAGCAATCGTGGCGAGCGCCGAGGAAGTCTTGAAATTCATTTCCGATCGAAAGCTGCCCGGATCG
GGTATCGGATATGTCGATGCACATTTGCTCGCCGCTGCCGCGCTCACGCCGGAAACGAGCGTCTGGACGCGCGACAAGCG
TCTGCTGGCTGCGGCTCAAGCGCTGTCGCTTGCCACCAAATTCCCGGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT168609 WP_002887278.1 NZ_CP059312:c4619713-4619447 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT168609 NZ_CP076135:c4794313-4794122 [Bradyrhizobium sediminis]
ATGAGAACCACCATCGCGATTGATGACGAATTGTTCGCCAAGGCGCAGGAATTTGCTGGAGTCACCGAGAAATCAGCGGT
CATCAGGGAAGCCTTGAAGGCGTTTGTCGAGCGCGAAGCTGCGCGGCGGCTCGCCCGCATGGGAGGGACGCAACCGGAAG
CAAAGGCTCCGCCGCGTCGACGCTTCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References