Detailed information of TA system TA168593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 209481..210003
• Replicon: plasmid pKp46596-1
• Accession: NZ_CP059311
• Organism: Klebsiella pneumoniae strain Kp46596

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: H1D28_RS02320
• Protein ID: WP_038991638.1
• Coordinates: 209719..210003 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: H1D28_RS02315
• Protein ID: WP_004181777.1
• Coordinates: 209481..209729 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D28_RS02295	204690..205511	-	822	WP_004181772.1	hypothetical protein	-
H1D28_RS02300	205573..205926	-	354	WP_004181774.1	hypothetical protein	-
H1D28_RS02305	206071..207057	-	987	WP_040120328.1	hypothetical protein	-
H1D28_RS02310	207391..209190	-	1800	WP_038991636.1	ATP-dependent helicase	-
H1D28_RS02315	209481..209729	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H1D28_RS02320	209719..210003	+	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1D28_RS02325	210020..210121	-	102	Protein_227	IS200/IS605 family transposase	-
H1D28_RS02330	210157..211383	+	1227	WP_040120327.1	transposase	-
H1D28_RS02335	211655..211879	-	225	Protein_229	transposase	-
H1D28_RS02340	211958..212386	-	429	WP_077256229.1	IS200/IS605 family transposase	-
H1D28_RS02345	212422..213609	+	1188	WP_040120325.1	transposase	-
H1D28_RS02350	213708..214803	-	1096	Protein_232	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / blaNDM-1 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / catA1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	htpB	1..286241	286241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T168593 WP_038991638.1 NZ_CP059311:209719-210003 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T168593 NZ_CP076123:3280348-3280725 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT168593 WP_004181777.1 NZ_CP059311:209481-209729 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT168593 NZ_CP076123:3279890-3280258 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8