Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 122199..123109 Replicon plasmid pKp46596-1
Accession NZ_CP059311
Organism Klebsiella pneumoniae strain Kp46596

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag H1D28_RS01835 Protein ID WP_004026354.1
Coordinates 122199..122669 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag H1D28_RS01840 Protein ID WP_004181895.1
Coordinates 122666..123109 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D28_RS01815 118399..118557 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
H1D28_RS01820 118629..119741 + 1113 WP_014386573.1 IS481-like element ISKpn28 family transposase -
H1D28_RS01830 121829..122089 + 261 WP_004026352.1 hypothetical protein -
H1D28_RS01835 122199..122669 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
H1D28_RS01840 122666..123109 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
H1D28_RS01845 123210..124481 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
H1D28_RS01850 124481..124903 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
H1D28_RS01855 125844..126875 - 1032 WP_170919122.1 CRISPR-associated protein Csf2 -
H1D28_RS01860 126880..127557 - 678 WP_071889217.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / blaNDM-1 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / catA1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 htpB 1..286241 286241
- flank IS/Tn - - 127771..129033 1262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T168592 WP_004026354.1 NZ_CP059311:c122669-122199 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T168592 NZ_CP076123:c3166551-3166144 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT168592 WP_004181895.1 NZ_CP059311:c123109-122666 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT168592 NZ_CP076123:c3166798-3166532 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References