Detailed information of TA system TA168591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 37662..38413
• Replicon: plasmid pKp46596-1
• Accession: NZ_CP059311
• Organism: Klebsiella pneumoniae strain Kp46596

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: H1D28_RS01415
• Protein ID: WP_014386536.1
• Coordinates: 37931..38413 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: H1D28_RS01410
• Protein ID: WP_004902250.1
• Coordinates: 37662..37940 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D28_RS01375	33305..33730	-	426	WP_000522996.1	organomercurial transporter MerC	-
H1D28_RS01380	33758..34033	-	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
H1D28_RS01385	34049..34414	-	366	WP_001294656.1	mercuric ion transporter MerT	-
H1D28_RS01390	34486..34941	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
H1D28_RS01395	35594..36052	+	459	WP_014386535.1	hypothetical protein	-
H1D28_RS01400	36882..37223	+	342	WP_004902257.1	hypothetical protein	-
H1D28_RS01405	37331..37543	+	213	WP_004902255.1	hypothetical protein	-
H1D28_RS01410	37662..37940	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
H1D28_RS01415	37931..38413	+	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
H1D28_RS01420	39448..39987	+	540	WP_004902239.1	hypothetical protein	-
H1D28_RS01425	40092..40484	+	393	WP_032442757.1	hypothetical protein	-
H1D28_RS01430	40585..41340	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
H1D28_RS01435	41367..42014	+	648	WP_014386537.1	EcsC family protein	-
H1D28_RS01440	42146..42415	-	270	Protein_50	kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / blaNDM-1 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / catA1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	htpB	1..286241	286241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T168591 WP_014386536.1 NZ_CP059311:37931-38413 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T168591 NZ_CP076123:c3011343-3011008 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT168591 WP_004902250.1 NZ_CP059311:37662-37940 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT168591 NZ_CP076123:c3011591-3011343 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3