Detailed information of TA system TA168588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 163006..163594
• Replicon: plasmid pAC1633-1
• Accession: NZ_CP059301
• Organism: Acinetobacter baumannii strain AC1633

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: H0529_RS21840
• Protein ID: WP_000438825.1
• Coordinates: 163307..163594 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: H0529_RS21835
• Protein ID: WP_004728120.1
• Coordinates: 163006..163320 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0529_RS21805	158106..158474	-	369	Protein_152	IS1-like element ISAba3 family transposase	-
H0529_RS21810	158555..159397	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
H0529_RS21815	159451..160479	+	1029	WP_081106503.1	transposase	-
H0529_RS21820	160594..161346	+	753	Protein_155	IS1-like element ISAba3 family transposase	-
H0529_RS21825	161474..162301	+	828	WP_000073658.1	AraC family transcriptional regulator	-
H0529_RS21830	162351..162956	+	606	WP_000447788.1	LysE family translocator	-
H0529_RS21835	163006..163320	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
H0529_RS21840	163307..163594	-	288	WP_000438825.1	BrnT family toxin	Toxin
H0529_RS21845	163866..164396	-	531	WP_000172759.1	hypothetical protein	-
H0529_RS21850	164551..164871	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H0529_RS21855	164864..165136	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
H0529_RS21860	165629..167104	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
H0529_RS21865	167160..168044	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	1..174292	174292
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	149082..170063	20981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T168588 WP_000438825.1 NZ_CP059301:c163594-163307 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T168588 NZ_CP076123:c2671061-2670759 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT168588 WP_004728120.1 NZ_CP059301:c163320-163006 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT168588 NZ_CP076123:c2670723-2670382 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGTCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9