Detailed information of TA system TA168568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 309335..309909
• Replicon: chromosome
• Accession: NZ_CP059297
• Organism: Brevundimonas sp. AJA228-03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HZ989_RS01645
• Protein ID: WP_209321916.1
• Coordinates: 309335..309700 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HZ989_RS01650
• Protein ID: WP_209321917.1
• Coordinates: 309694..309909 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ989_RS01620 (HZ989_01620)	305005..306180	-	1176	WP_209323011.1	aspartate aminotransferase family protein	-
HZ989_RS01625 (HZ989_01625)	306406..307227	-	822	WP_209321912.1	ABC transporter permease	-
HZ989_RS01630 (HZ989_01630)	307372..308766	-	1395	WP_209321913.1	dihydrolipoyl dehydrogenase	-
HZ989_RS01635 (HZ989_01635)	308770..308943	-	174	WP_209321914.1	hypothetical protein	-
HZ989_RS01640 (HZ989_01640)	309201..309332	-	132	WP_209321915.1	2-oxo acid dehydrogenase subunit E2	-
HZ989_RS01645 (HZ989_01645)	309335..309700	-	366	WP_209321916.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZ989_RS01650 (HZ989_01650)	309694..309909	-	216	WP_209321917.1	ribbon-helix-helix protein, CopG family	Antitoxin
HZ989_RS01655 (HZ989_01655)	309971..311713	-	1743	WP_209321918.1	2-oxo acid dehydrogenase subunit E2	-
HZ989_RS01660 (HZ989_01660)	311713..312840	-	1128	WP_209321919.1	alpha-ketoacid dehydrogenase subunit beta	-
HZ989_RS01665 (HZ989_01665)	312988..314220	-	1233	WP_209321920.1	3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) subunit alpha	-
HZ989_RS01670 (HZ989_01670)	314365..314856	+	492	WP_209321921.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	304049..305008	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13437.21 Da
• Isoelectric Point: 4.5551

>T168568 WP_209321916.1 NZ_CP059297:c309700-309335 [Brevundimonas sp. AJA228-03]
MVKALFDTNILIDHLNAAPEADAEIDRYDDRAISIISWIEVMAGAGPDLAEPTRTYLDGYQIVGLEDRIAEQAASLRQSR
RLKLPDAVIWATAQTTGRLLVTRNTKDFPADDPGVRAPYRI

Nucleotide

• Length: 366 bp

>T168568 NZ_CP076116:1521232-1521576 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAGCAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGCTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7999.02 Da
• Isoelectric Point: 8.5613

>AT168568 WP_209321917.1 NZ_CP059297:c309909-309694 [Brevundimonas sp. AJA228-03]
MRILVDANEDQVAALDALAKREKRPRAAVIRAAIDDYVARNKRQAVKDGFGLWGQGGEDGLAYQERLRSEW

Nucleotide

• Length: 216 bp

>AT168568 NZ_CP076116:1521573-1521884 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA