Detailed information of TA system TA168567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 152816..153359
• Replicon: chromosome
• Accession: NZ_CP059297
• Organism: Brevundimonas sp. AJA228-03

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HZ989_RS00795
• Protein ID: WP_209321760.1
• Coordinates: 153099..153359 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HZ989_RS00790
• Protein ID: WP_209321759.1
• Coordinates: 152816..153070 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ989_RS00775 (HZ989_00775)	148909..150879	+	1971	WP_209321757.1	M1 family metallopeptidase	-
HZ989_RS00780 (HZ989_00780)	150942..152609	+	1668	WP_209321758.1	amidohydrolase	-
HZ989_RS00790 (HZ989_00790)	152816..153070	-	255	WP_209321759.1	BrnA antitoxin family protein	Antitoxin
HZ989_RS00795 (HZ989_00795)	153099..153359	-	261	WP_209321760.1	BrnT family toxin	Toxin
HZ989_RS00800 (HZ989_00800)	153565..154533	-	969	WP_209321761.1	TraB/GumN family protein	-
HZ989_RS00805 (HZ989_00805)	154593..155183	-	591	WP_209321762.1	hypothetical protein	-
HZ989_RS00810 (HZ989_00810)	155180..155383	-	204	WP_209321763.1	helix-turn-helix transcriptional regulator	-
HZ989_RS00815 (HZ989_00815)	155388..155852	-	465	WP_209321764.1	hypothetical protein	-
HZ989_RS00820 (HZ989_00820)	155932..157155	-	1224	WP_209321765.1	DNA recombination protein RmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (7-80)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9831.23 Da
• Isoelectric Point: 9.1014

>T168567 WP_209321760.1 NZ_CP059297:c153359-153099 [Brevundimonas sp. AJA228-03]
MDIEFDAAKDAANIARHGVSLALGACMDMDTAVIDRDDRRDYGEVRFNALGRIEGRVYAMTFTVRDAIRIISLRKANPRE
QRRFVR

Nucleotide

• Length: 261 bp

>T168567 NZ_CP076115:c3252153-3251740 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTAATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9279.56 Da
• Isoelectric Point: 10.4367

>AT168567 WP_209321759.1 NZ_CP059297:c153070-152816 [Brevundimonas sp. AJA228-03]
MTDDDTTPEFTDAVMARARPAREVLSPEVMEAFRNKGGRPRAVAAKVPVSLRLDPDVVEAWKATGTGWQTRINDALRRAM
PRAS

Nucleotide

• Length: 255 bp

>AT168567 NZ_CP076115:c3252373-3252140 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA