Detailed information of TA system TA168551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1678412..1678998
• Replicon: chromosome
• Accession: NZ_CP059295
• Organism: Klebsiella pneumoniae strain ER17974.3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7Z9B6M3
• Locus tag: H0490_RS07905
• Protein ID: WP_029884514.1
• Coordinates: 1678630..1678998 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: H0490_RS07900
• Protein ID: WP_004174006.1
• Coordinates: 1678412..1678633 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0490_RS07880 (1674569)	1674569..1675495	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H0490_RS07885 (1675492)	1675492..1676769	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
H0490_RS07890 (1676766)	1676766..1677533	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H0490_RS07895 (1677535)	1677535..1678248	+	714	WP_029884513.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H0490_RS07900 (1678412)	1678412..1678633	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H0490_RS07905 (1678630)	1678630..1678998	+	369	WP_029884514.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H0490_RS07910 (1679271)	1679271..1680587	+	1317	WP_029884515.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H0490_RS07915 (1680694)	1680694..1681581	+	888	WP_004901104.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H0490_RS07920 (1681578)	1681578..1682423	+	846	WP_004901101.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H0490_RS07925 (1682425)	1682425..1683495	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1637078..1684232	47154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13583.01 Da
• Isoelectric Point: 8.6410

>T168551 WP_029884514.1 NZ_CP059295:1678630-1678998 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T168551 NZ_CP076104:3567057-3567458 [Nocardioides sp. LMS-CY]
GTGATCGTCCTCGACACGTCTGCTGCGATCGAGTGGCTCCTCGTGCGTGACCTCGGAGCGGCTGTCGCCGCTCGGTTCAG
CGACCCCGATGTCACCGTGCACGCGCCGTCACTGATCGGCGTCGAGGTCGCGGCAGCCCTGCGGGGCCTCGTGCTCGGTG
GGCACGCGCAGCCTGAGCGGGCATCGCTCGCACTAGCGGACCTGACCGCGGCGGACATCACTCTTCATGATCCGACCCCG
CTTCTGCCCCGAGCTTGGGAGTTGCGGGACAACCTCACCCCGTACGACGCGGTGTACCTCGCGCTTGCCGAGGTCCTCGA
CGCGAGGCTGGTCACCACTGACGCACGCATTGCCACGGCGCCGGGGCTGCGGGCCGAGATCGACCTGGTCACGAGCCCCT
GA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT168551 WP_004174006.1 NZ_CP059295:1678412-1678633 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT168551 NZ_CP076104:3566833-3567060 [Nocardioides sp. LMS-CY]
ATGACGATGATCCAGATCCGCAACGTGCCCGACGACGTCCACCGACGCCTCAAGGCACGTGCGGCCCTCGAGGGCGTCTC
GCTGTCCGACCTGGCACTTGCCGAGTTGCGGCGGTCCTTGGACCGGCCGACTCGTCGTGAGCTTCTCGAGCTGGTGGCCT
CCCGTGAGCCTGTCCGCTCCGGCCCTTCCTCGCCGGAGTTGATCGACTCGGGACGCAACGCCCCGTGA

Structures

Toxin

• AlphaFold DB: A0A7Z9B6M3

Antitoxin

• AlphaFold DB: A0A5E5YJY7