Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1547157..1547896 Replicon chromosome
Accession NZ_CP059295
Organism Klebsiella pneumoniae strain ER17974.3

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag H0490_RS07350 Protein ID WP_004901283.1
Coordinates 1547411..1547896 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H0490_RS07345 Protein ID WP_003026799.1
Coordinates 1547157..1547423 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0490_RS07330 (1542661) 1542661..1544730 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
H0490_RS07335 (1545026) 1545026..1546395 + 1370 WP_087661561.1 IS3 family transposase -
H0490_RS07340 (1546596) 1546596..1547024 + 429 WP_004901287.1 GFA family protein -
H0490_RS07345 (1547157) 1547157..1547423 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H0490_RS07350 (1547411) 1547411..1547896 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
H0490_RS07355 (1548221) 1548221..1549267 - 1047 WP_022615678.1 IS481-like element ISKpn28 family transposase -
H0490_RS07360 (1549341) 1549341..1549493 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
H0490_RS07365 (1549795) 1549795..1551414 + 1620 WP_029884695.1 ATP-binding cassette domain-containing protein -
H0490_RS07370 (1551513) 1551513..1551725 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
H0490_RS07375 (1551977) 1551977..1552267 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 1545670..1549267 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T168550 WP_004901283.1 NZ_CP059295:1547411-1547896 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T168550 NZ_CP076104:c2702262-2702017 [Nocardioides sp. LMS-CY]
ATGCCCGTCACGGCGAGTGAGGCCCGCAAGCAGCTGTTTCCGCTGATCGAGAAGGTCAACAACGACCGCATCGCGGTCGA
GATCACCTCGCGCCGTGGCAATGCCGTCTTGATCTCGGCCGAGGAGTTCGCGGCTCTGGAGGAGACGGCCTACCTGTTGC
GGGTGCCTGCCAACGCCAAGCGGCTTCTCGAGAGCCTTCAGCAGGCGAGGTCGGGCGATCGCCACGAGCACGAGCTCGCC
GAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT168550 WP_003026799.1 NZ_CP059295:1547157-1547423 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT168550 NZ_CP076104:c2702020-2701763 [Nocardioides sp. LMS-CY]
GTGAGGCTCGTCTTCACACCGAACGGCTGGGACGACTACCAACACTGGCTGCAGGCCGACCGGCAGATTCTCAAGCGGAT
CAACCGGCTGATCGATGACGCGCTGAGAGACCCGACCGACGGCATCGGCAAGCCGGAGCCGTTGAGGCACATGTTCGCTG
GTGCGTGGTCTCGCCGCATCACCGACGAGCACCGCCTGGTGTACCTGGTCGATGGCGAGGACCTCGTCGTCCTTCAGGCA
CGCTTCCACTACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References