Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1267820..1268445 Replicon chromosome
Accession NZ_CP059295
Organism Klebsiella pneumoniae strain ER17974.3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H0490_RS06010 Protein ID WP_002882817.1
Coordinates 1267820..1268203 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H0490_RS06015 Protein ID WP_004150355.1
Coordinates 1268203..1268445 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0490_RS05995 (1265186) 1265186..1266088 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H0490_RS06000 (1266085) 1266085..1266720 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H0490_RS06005 (1266717) 1266717..1267646 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H0490_RS06010 (1267820) 1267820..1268203 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H0490_RS06015 (1268203) 1268203..1268445 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
H0490_RS06020 (1268650) 1268650..1269567 + 918 WP_029884108.1 alpha/beta hydrolase -
H0490_RS06025 (1269581) 1269581..1270522 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H0490_RS06030 (1270567) 1270567..1271004 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
H0490_RS06035 (1271001) 1271001..1271861 - 861 WP_002882807.1 virulence factor BrkB family protein -
H0490_RS06040 (1271855) 1271855..1272454 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T168549 WP_002882817.1 NZ_CP059295:c1268203-1267820 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T168549 NZ_CP076104:c2702020-2701763 [Nocardioides sp. LMS-CY]
GTGAGGCTCGTCTTCACACCGAACGGCTGGGACGACTACCAACACTGGCTGCAGGCCGACCGGCAGATTCTCAAGCGGAT
CAACCGGCTGATCGATGACGCGCTGAGAGACCCGACCGACGGCATCGGCAAGCCGGAGCCGTTGAGGCACATGTTCGCTG
GTGCGTGGTCTCGCCGCATCACCGACGAGCACCGCCTGGTGTACCTGGTCGATGGCGAGGACCTCGTCGTCCTTCAGGCA
CGCTTCCACTACAAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT168549 WP_004150355.1 NZ_CP059295:c1268445-1268203 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT168549 NZ_CP076104:c2702262-2702017 [Nocardioides sp. LMS-CY]
ATGCCCGTCACGGCGAGTGAGGCCCGCAAGCAGCTGTTTCCGCTGATCGAGAAGGTCAACAACGACCGCATCGCGGTCGA
GATCACCTCGCGCCGTGGCAATGCCGTCTTGATCTCGGCCGAGGAGTTCGCGGCTCTGGAGGAGACGGCCTACCTGTTGC
GGGTGCCTGCCAACGCCAAGCGGCTTCTCGAGAGCCTTCAGCAGGCGAGGTCGGGCGATCGCCACGAGCACGAGCTCGCC
GAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References