168545

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 13170..13635 Replicon plasmid p1506-3-mcr
Accession NZ_CP059291
Organism Escherichia coli strain 1506

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag H0481_RS23350 Protein ID WP_001230707.1
Coordinates 13384..13635 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag H0481_RS23345 Protein ID WP_000801440.1
Coordinates 13170..13397 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0481_RS23305 8400..8615 - 216 WP_000760375.1 EexN family lipoprotein -
H0481_RS23310 8612..8905 - 294 WP_060598542.1 LuxR family transcriptional regulator -
H0481_RS23315 8982..10211 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
H0481_RS23320 10215..10718 - 504 WP_000781813.1 hypothetical protein -
H0481_RS23325 11079..11426 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
H0481_RS23330 11423..11671 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
H0481_RS23335 11976..12326 - 351 WP_000854259.1 hypothetical protein -
H0481_RS23340 12417..12716 - 300 WP_000650309.1 hypothetical protein -
H0481_RS23345 13170..13397 - 228 WP_000801440.1 hypothetical protein Antitoxin
H0481_RS23350 13384..13635 - 252 WP_001230707.1 hypothetical protein Toxin
H0481_RS23355 13632..14174 - 543 WP_001083906.1 hypothetical protein -
H0481_RS23360 14279..14794 - 516 WP_001025395.1 J domain-containing protein -
H0481_RS23365 16209..17075 + 867 WP_001353743.1 replication initiation protein -
H0481_RS23370 17088..17492 + 405 WP_012421203.1 hypothetical protein -
H0481_RS23375 17627..18154 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-55)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T168545 WP_001230707.1 NZ_CP059291:c13635-13384 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T168545 NZ_CP076082:c1059420-1059157 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAGAAATTAGTACCGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGTACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATTACGAATAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT168545 WP_000801440.1 NZ_CP059291:c13397-13170 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT168545 NZ_CP076082:c1059677-1059417 [Limosilactobacillus fermentum]
ATGGACAAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAGGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAACGGGAAAACGAGCCGCTCGACGACTTTGAT
GATGTTTGGGGTCAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References