Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3392719..3393337 Replicon chromosome
Accession NZ_CP059288
Organism Escherichia coli strain 1506

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H0481_RS16455 Protein ID WP_001291435.1
Coordinates 3393119..3393337 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H0481_RS16450 Protein ID WP_000344800.1
Coordinates 3392719..3393093 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0481_RS16440 3387808..3389001 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H0481_RS16445 3389024..3392173 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H0481_RS16450 3392719..3393093 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H0481_RS16455 3393119..3393337 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H0481_RS16460 3393509..3394060 + 552 WP_000102564.1 maltose O-acetyltransferase -
H0481_RS16465 3394176..3394646 + 471 WP_000136192.1 YlaC family protein -
H0481_RS16470 3394810..3396360 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H0481_RS16475 3396402..3396755 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H0481_RS16485 3397134..3397445 + 312 WP_162000861.1 MGMT family protein -
H0481_RS16490 3397476..3398048 - 573 WP_063117855.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168539 WP_001291435.1 NZ_CP059288:3393119-3393337 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T168539 NZ_CP076049:c1203014-1202835 [Cupriavidus metallidurans]
TTGCATTCGTCCACGCTGATCCGATTGCTCGAACAGAAGGGGTGGAAGCTGGTGCGCACGCATGGCAGTCATTTCACCTT
CAAACATCCGCGGCACATGCTGATTGTGACCGTGCCCCATCCAAAGAAGGATCTGCCGACAGGCACGGTCCGAAACATTC
TGAGGACGGCCGGGCTATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT168539 WP_000344800.1 NZ_CP059288:3392719-3393093 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT168539 NZ_CP076049:c1202805-1202401 [Cupriavidus metallidurans]
ATGGACTTTCCCATTGCCATTCACAAGGATGCGAACTCGGTCTACGGCGTGACGGTGCCCGATGTGGCGGGCTGTTACTC
CGCCGGCGAATCCATCGAGGAAGCGGTCCGCAACGCACGCGAGGCCATCACGACTCACGTGGTGGCCCTGCTCTCCCTTG
GCGAGGAGGTTGTTCTGAAAGCGACGCCGATCGGCACGCTGATGGCCCAACCGGAGTACGCAGGCGCGATCTGGGCGCTG
GCCGACATCGACATGAGCAAGCTCGACGCACGCCCGGAGCGAATCAATATCAGCCTGCCTCGTTTTGTATTGCACAAGAT
CGACACGTTCGTGGAACGTCGCCACGAAACCCGGAGCGGGTTCCTCGCGCGCGTCGCGCTGGACGCCATCGCGGGCAGCG
TCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References