Detailed information of TA system TA168538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2399659..2400297
• Replicon: chromosome
• Accession: NZ_CP059288
• Organism: Escherichia coli strain 1506

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: H0481_RS11640
• Protein ID: WP_000813794.1
• Coordinates: 2400121..2400297 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H0481_RS11635
• Protein ID: WP_001270286.1
• Coordinates: 2399659..2400075 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0481_RS11615	2394811..2395752	-	942	WP_001251313.1	ABC transporter permease	-
H0481_RS11620	2395753..2396766	-	1014	WP_113418672.1	ABC transporter ATP-binding protein	-
H0481_RS11625	2396784..2397929	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H0481_RS11630	2398174..2399580	-	1407	WP_113418670.1	PLP-dependent aminotransferase family protein	-
H0481_RS11635	2399659..2400075	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H0481_RS11640	2400121..2400297	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H0481_RS11645	2400519..2400749	+	231	WP_000494244.1	YncJ family protein	-
H0481_RS11650	2400841..2402802	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H0481_RS11655	2402875..2403411	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
H0481_RS11660	2403464..2404678	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2404718..2405983	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T168538 WP_000813794.1 NZ_CP059288:c2400297-2400121 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T168538 NZ_CP076048:3923900-3924505 [Rhodococcus sp. USK10]
GTGAAACGACTCGACCTGGTGGTCGGCTGCGACGGCGCCGGCAAGTCCACCTTCGTGGCGCTCACCCTTGCTCCCCTGCT
GCCGGCCAGCCCGTTAGTCAATGCCGACGAGATCGCCAGACAACGATGGCCGGCGAATCCGGCTGCACACTCCTACGCGG
CGGCAGAGATCGCCGCGAAGACCCGATCACACCTATTGAGCCGGGGAATGTCGTTCATCGCCGAGACGGTGTTCTCCCAT
CCGTCGAAGCTGGAGCTGATCGACGAAGCGCGCACGCACGAGTACACCGTCGTCTTGCATGTGGTCATTATTCCCGAGGA
GCTAGCGGTCCGGCGTGTGCGCTATCGGGTGCGAGCCGGCGGACACGATGTGCCGGAAGACAAGATCCGCCAGCGATACC
AAAGATTGTGGGGTCTCGTCGCTCAAGCGTTCCCACGTTGTGATCACGTGACCGTGTACGACAACAGCGCGAGCACCGGC
CCGCGCATCGTCGCTCAGTTCACCGACGGATACCTCGTCGGCGAAGCCAACTGGCCCTCCTGGACACCCACAGAACTGAC
CGCGAGCACCAGCCCGGGCGCACAACCAAAGGACCCGAACGTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT168538 WP_001270286.1 NZ_CP059288:c2400075-2399659 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT168538 NZ_CP076048:3923520-3923903 [Rhodococcus sp. USK10]
ATGCCGAGTAGTGGAGACAAGGTCACCCGCCTGGCAGCAGACCTGGTAGCCAGCGCCATGGCCGAAGGAGAACGGCAGCA
CCGCACAGGACGTCAGCAACTCGAACACTGGGCCCGGGTGGGACGGGCGGTCTCGAGCCGGCAGACCGCGGCCCGGCGCC
GAGTAGAGGAAGCCCTCGCCGGCCGGTTGCCGATCGATGAACTCGGAACCGAAGAAGGCACAGTTTTCAACGCCGAGATC
AACGCGGCGATCGAGGAACAACTGGCCACCACCGACTACGGCGCCGCGCTCGCGGCCCAGGGAGTCACCACGGTCGCGTT
GTCGGAGGACGGTCAGATCATCGAGTACCGTCCCGACGGCACCCACGCAGTCCTGCCCGAGTGA

Structures

Toxin

• PDB: 6HPB