Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 880829..881483 Replicon chromosome
Accession NZ_CP059288
Organism Escherichia coli strain 1506

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H0481_RS04240 Protein ID WP_000244777.1
Coordinates 881076..881483 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H0481_RS04235 Protein ID WP_000354046.1
Coordinates 880829..881095 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0481_RS04210 875998..876741 + 744 WP_000951964.1 SDR family oxidoreductase -
H0481_RS04215 876798..878231 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H0481_RS04220 878276..878587 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H0481_RS04225 878751..879410 + 660 WP_181463993.1 hemolysin III family protein -
H0481_RS04230 879606..880586 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H0481_RS04235 880829..881095 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H0481_RS04240 881076..881483 + 408 WP_000244777.1 toxin CptA Toxin
H0481_RS04245 881523..882044 - 522 WP_001055874.1 flavodoxin FldB -
H0481_RS04250 882156..883052 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H0481_RS04255 883077..883787 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H0481_RS04260 883793..885526 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T168527 WP_000244777.1 NZ_CP059288:881076-881483 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T168527 NZ_CP076043:4244663-4245070 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT168527 WP_000354046.1 NZ_CP059288:880829-881095 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT168527 NZ_CP076043:4244416-4244682 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References