Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3473658..3474276 Replicon chromosome
Accession NZ_CP059283
Organism Escherichia coli strain SY3626_hybrid

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H0155_RS17075 Protein ID WP_001291435.1
Coordinates 3474058..3474276 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H0155_RS17070 Protein ID WP_000344800.1
Coordinates 3473658..3474032 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0155_RS17060 3468747..3469940 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H0155_RS17065 3469963..3473112 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H0155_RS17070 3473658..3474032 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H0155_RS17075 3474058..3474276 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H0155_RS17080 3474448..3474999 + 552 WP_000102564.1 maltose O-acetyltransferase -
H0155_RS17085 3475115..3475585 + 471 WP_000136192.1 YlaC family protein -
H0155_RS17090 3475749..3477299 + 1551 WP_176580736.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H0155_RS17095 3477341..3477694 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H0155_RS17105 3478073..3478384 + 312 WP_000409911.1 MGMT family protein -
H0155_RS17110 3478415..3478987 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168510 WP_001291435.1 NZ_CP059283:3474058-3474276 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T168510 NZ_CP076043:732399-732650 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT168510 WP_000344800.1 NZ_CP059283:3473658-3474032 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT168510 NZ_CP076043:732647-732901 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References