Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4828380..4829180 Replicon chromosome
Accession NZ_CP059279
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag H0271_RS23330 Protein ID WP_000342452.1
Coordinates 4828380..4828907 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag H0271_RS23335 Protein ID WP_001277107.1
Coordinates 4828914..4829180 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0271_RS23305 4823456..4824223 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
H0271_RS23310 4824220..4825497 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
H0271_RS23315 4825494..4826420 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H0271_RS23320 4826468..4827577 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
H0271_RS23325 4828000..4828383 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
H0271_RS23330 4828380..4828907 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
H0271_RS23335 4828914..4829180 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
H0271_RS23340 4829329..4830432 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
H0271_RS23345 4830703..4831557 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
H0271_RS23350 4831802..4832860 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
H0271_RS23355 4832853..4833521 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T168454 WP_000342452.1 NZ_CP059279:c4828907-4828380 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T168454 NZ_CP076008:338066-338434 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAAATGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT168454 WP_001277107.1 NZ_CP059279:c4829180-4828914 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT168454 NZ_CP076008:337848-338069 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References