Detailed information of TA system TA168451

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4301490..4302324
• Replicon: chromosome
• Accession: NZ_CP059279
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H0271_RS20735
• Protein ID: WP_001546109.1
• Coordinates: 4301947..4302324 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: H0271_RS20730
• Protein ID: WP_001546108.1
• Coordinates: 4301490..4301870 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0271_RS20695	4297770..4298225	+	456	WP_001545736.1	hypothetical protein	-
H0271_RS20700	4298304..4298537	+	234	WP_001119729.1	DUF905 family protein	-
H0271_RS20705	4298637..4299455	+	819	WP_001234620.1	DUF945 domain-containing protein	-
H0271_RS20710	4299510..4299995	+	486	WP_000849588.1	antirestriction protein	-
H0271_RS20715	4300011..4300487	+	477	WP_001424026.1	RadC family protein	-
H0271_RS20720	4300556..4300777	+	222	WP_000692345.1	DUF987 domain-containing protein	-
H0271_RS20725	4300796..4301440	+	645	WP_000086755.1	hypothetical protein	-
H0271_RS20730	4301490..4301870	+	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H0271_RS20735	4301947..4302324	+	378	WP_001546109.1	TA system toxin CbtA family protein	Toxin
H0271_RS20740	4302321..4302808	+	488	Protein_4047	hypothetical protein	-
H0271_RS20745	4302825..4303022	+	198	WP_042024643.1	DUF957 domain-containing protein	-
H0271_RS20750	4303107..4303952	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
H0271_RS20755	4304021..4304416	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
H0271_RS20760	4304409..4305342	+	934	Protein_4051	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
H0271_RS20765	4305774..4305929	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT	4301490..4319900	18410
	-	flank	IS/Tn	-	-	4305774..4305929	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.00 Da
• Isoelectric Point: 8.5190

>T168451 WP_001546109.1 NZ_CP059279:4301947-4302324 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYKMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T168451 NZ_CP075897:c1531597-1531319 [Exiguobacterium acetylicum]
ATGACAGCATATACGGTGGAGTTTGAGCGCGGAGCCCAAAAGTCTCTCAAGAAGATGGATCCCCAACAAGCGCGGATTAT
CATGTCCTGGATCAAGAATAATCTGGTCGGAACGGATGATCCGCGTCGTCATGGTAGAGGGCTCGTCTCGAACAGATCGG
GTGAATGGCGTTATCGCATTGGCGACTATCGTCTTATTGCTGACATCCAGGATGAAAAAGTCGTGATCTTGATTCTTGAA
ATTGGACATCGTCGTGATATCTATAAATATGGCAGTTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT168451 WP_001546108.1 NZ_CP059279:4301490-4301870 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT168451 NZ_CP075897:c1531818-1531594 [Exiguobacterium acetylicum]
ATGAGTACCATTTCCGTACGTCTGGATGATCAGGATACACGACTCATCAAGGAATATGCGAAAGCAAAGAACATCACGAT
TTCTACACTCGTTCGCGATGCTGTCCTCGACCGCATCGAAGACGAAATCGATTTGCAACTCTATCATGATTCGATGGCAG
CACACCGTAAAAAATCAGAAGCGATCTCTTTCGGCGATATGATGAAGGAACTTGATTTAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8