Detailed information of TA system TA168448

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3118687..3119518
• Replicon: chromosome
• Accession: NZ_CP059279
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: H0271_RS15230
• Protein ID: WP_000854814.1
• Coordinates: 3119144..3119518 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A641DSP2
• Locus tag: H0271_RS15225
• Protein ID: WP_001546021.1
• Coordinates: 3118687..3119055 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0271_RS15190	3113744..3114814	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
H0271_RS15195	3114950..3115627	+	678	WP_001362823.1	hypothetical protein	-
H0271_RS15200	3115643..3116053	+	411	WP_000846704.1	hypothetical protein	-
H0271_RS15205	3116274..3117095	+	822	WP_001234710.1	DUF945 domain-containing protein	-
H0271_RS15210	3117358..3117831	+	474	WP_001385393.1	antirestriction protein	-
H0271_RS15215	3117847..3118323	+	477	WP_001186773.1	RadC family protein	-
H0271_RS15220	3118386..3118607	+	222	WP_000692323.1	DUF987 domain-containing protein	-
H0271_RS15225	3118687..3119055	+	369	WP_001546021.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H0271_RS15230	3119144..3119518	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
H0271_RS15235	3119515..3119709	+	195	WP_000988601.1	hypothetical protein	-
H0271_RS15240	3119722..3119835	+	114	WP_001161660.1	DUF957 domain-containing protein	-
H0271_RS15245	3120198..3120944	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H0271_RS15250	3120959..3122500	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H0271_RS15255	3122711..3122857	-	147	Protein_2974	transposase domain-containing protein	-
H0271_RS15260	3122911..3123093	+	183	WP_001016348.1	hypothetical protein	-
H0271_RS15265	3123194..3123523	-	330	WP_000450409.1	DUF496 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T168448 WP_000854814.1 NZ_CP059279:3119144-3119518 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T168448 NZ_CP075892:c93206-92901 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13607.47 Da
• Isoelectric Point: 6.3139

>AT168448 WP_001546021.1 NZ_CP059279:3118687-3119055 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT168448 NZ_CP075892:c93424-93206 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A641DSP2