Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1095518..1096068 Replicon chromosome
Accession NZ_CP059279
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H0271_RS05160 Protein ID WP_001160966.1
Coordinates 1095754..1096068 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H0271_RS05155 Protein ID WP_000125566.1
Coordinates 1095518..1095751 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0271_RS05130 1090591..1090878 + 288 WP_000203741.1 ferredoxin-like protein FixX -
H0271_RS05135 1090937..1092268 + 1332 WP_001183207.1 MFS transporter -
H0271_RS05140 1092376..1092906 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H0271_RS05145 1092899..1094761 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
H0271_RS05150 1094953..1095432 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
H0271_RS05155 1095518..1095751 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H0271_RS05160 1095754..1096068 + 315 WP_001160966.1 CcdB family protein Toxin
H0271_RS05165 1096065..1096913 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H0271_RS05170 1096920..1097297 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H0271_RS05175 1097300..1098121 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H0271_RS05180 1098118..1099107 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H0271_RS05185 1099107..1100393 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T168430 WP_001160966.1 NZ_CP059279:1095754-1096068 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T168430 NZ_CP075885:c1000615-1000376 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT168430 WP_000125566.1 NZ_CP059279:1095518-1095751 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT168430 NZ_CP075885:c1000847-1000605 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References