Detailed information of TA system TA168427

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 914667..915501
• Replicon: chromosome
• Accession: NZ_CP059279
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: H0271_RS04265
• Protein ID: WP_000854770.1
• Coordinates: 915124..915501 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: H0271_RS04260
• Protein ID: WP_001280950.1
• Coordinates: 914667..915035 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0271_RS04230	909819..911720	+	1902	Protein_812	autotransporter adhesin Ag43	-
H0271_RS04235	911791..911949	+	159	WP_001323397.1	DUF905 family protein	-
H0271_RS04240	912104..912922	+	819	WP_001234712.1	DUF945 domain-containing protein	-
H0271_RS04245	913264..913728	+	465	WP_000855061.1	antirestriction protein	-
H0271_RS04250	913744..914220	+	477	WP_001186779.1	RadC family protein	-
H0271_RS04255	914283..914504	+	222	WP_001220314.1	DUF987 domain-containing protein	-
H0271_RS04260	914667..915035	+	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H0271_RS04265	915124..915501	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
H0271_RS04270	915498..915986	+	489	WP_000761690.1	hypothetical protein	-
H0271_RS04275	916003..916179	+	177	WP_000839286.1	DUF957 domain-containing protein	-
H0271_RS04280	916279..916437	+	159	WP_001467148.1	hypothetical protein	-
H0271_RS04285	916801..916977	+	177	Protein_823	helix-turn-helix domain-containing protein	-
H0271_RS04290	917750..919390	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	866664..927147	60483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T168427 WP_000854770.1 NZ_CP059279:915124-915501 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T168427 NZ_CP075882:188402-188713 [Klebsiella michiganensis]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT168427 WP_001280950.1 NZ_CP059279:914667-915035 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT168427 NZ_CP075882:188710-189129 [Klebsiella michiganensis]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885