Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2030908..2031422 Replicon chromosome
Accession NZ_CP059180
Organism Staphylococcus aureus strain SH1000

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag SAURSH1000_RS09905 Protein ID WP_000581792.1
Coordinates 2030908..2031117 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SAURSH1000_RS09910 Protein ID WP_000138932.1
Coordinates 2031129..2031422 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAURSH1000_RS09885 (SAURSH1000_001976) 2026416..2027774 - 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SAURSH1000_RS09890 (SAURSH1000_001977) 2027789..2028859 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
SAURSH1000_RS09895 (SAURSH1000_001978) 2029177..2030379 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
SAURSH1000_RS09900 (SAURSH1000_001979) 2030643..2030780 - 138 WP_000828354.1 Lmo0850 family protein -
SAURSH1000_RS09905 (SAURSH1000_001980) 2030908..2031117 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
SAURSH1000_RS09910 (SAURSH1000_001981) 2031129..2031422 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SAURSH1000_RS09915 (SAURSH1000_001982) 2031588..2033072 + 1485 WP_000571549.1 cardiolipin synthase -
SAURSH1000_RS09920 (SAURSH1000_001983) 2033100..2033747 + 648 WP_001187629.1 HD domain-containing protein -
SAURSH1000_RS09925 (SAURSH1000_001984) 2034560..2035432 - 873 WP_000725802.1 membrane protein insertase YidC -
SAURSH1000_RS09930 (SAURSH1000_001985) 2035518..2036159 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T168419 WP_000581792.1 NZ_CP059180:c2031117-2030908 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T168419 NZ_CP075881:1748665-1749039 [Klebsiella michiganensis]
ATGAAATGGGTGAGCGCAAGCGAAGTCATTGCCTTTCATGATCGCATTTTACAACATCTCCCCGGCGTTAAAGGTATGTC
CGATCCTGGTCGGGCGGAAGCCATAATTTATCGTGTACAGAACCGCTTTCACTATGAAGGGGTGAACGACATTTTTGAAC
TGGCTGCAACCTATTGGGTCGCTATCGCGCGTGGGCACATTTTTAATGATGGTAATAAGAGGACTGCTTTTTTTATCACC
ATGACGTTCCTGGCCCGCAATGGGTATCTTATCGCTGATGAAGATACCCGGTTGGAAGAACTCACTGTGCTGGCTGCGAC
AGGCGAAGCTACGGTAGTGGTTCTTGCTGATGCGTTACGGCAATTGGCGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT168419 WP_000138932.1 NZ_CP059180:c2031422-2031129 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT168419 NZ_CP075881:1748447-1748668 [Klebsiella michiganensis]
ATGCGCTCATACAGTACATCTGAAGCCAGACAGAATATTGCGGCCGTCATGGACGCCGCTGCCAGCGGAGAACCGATTGA
AATTACGCGGCGTGATGGAACGTCTAATGTTTTGATCAGTAAGGCGGAATATGAAACGTTTATGAATGCCAAACTTGATG
CGGAGTTTGATTTTATTATGCAGCGACACGGCCGCACAGTTAAGGCGTTGACCGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References