Detailed information of TA system TA168418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2011151..2011680
• Replicon: chromosome
• Accession: NZ_CP059180
• Organism: Staphylococcus aureus strain SH1000

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAURSH1000_RS09815
• Protein ID: WP_000621175.1
• Coordinates: 2011151..2011513 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAURSH1000_RS09820
• Protein ID: WP_000948331.1
• Coordinates: 2011510..2011680 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAURSH1000_RS09795 (SAURSH1000_001958)	2008129..2008899	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
SAURSH1000_RS09800 (SAURSH1000_001959)	2008874..2009353	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAURSH1000_RS09805 (SAURSH1000_001960)	2009355..2009681	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAURSH1000_RS09810 (SAURSH1000_001961)	2009800..2010801	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAURSH1000_RS09815 (SAURSH1000_001962)	2011151..2011513	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAURSH1000_RS09820 (SAURSH1000_001963)	2011510..2011680	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAURSH1000_RS09825 (SAURSH1000_001964)	2011765..2012913	-	1149	WP_001281145.1	alanine racemase	-
SAURSH1000_RS09830 (SAURSH1000_001965)	2012979..2013338	-	360	WP_000581200.1	holo-ACP synthase	-
SAURSH1000_RS09835 (SAURSH1000_001966)	2013342..2013833	-	492	WP_001205910.1	PH domain-containing protein	-
SAURSH1000_RS09840 (SAURSH1000_001967)	2013820..2015403	-	1584	WP_001294626.1	PH domain-containing protein	-
SAURSH1000_RS09845 (SAURSH1000_001968)	2015396..2015875	-	480	WP_001287088.1	hypothetical protein	-
SAURSH1000_RS09850 (SAURSH1000_001969)	2016084..2016644	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T168418 WP_000621175.1 NZ_CP059180:c2011513-2011151 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T168418 NZ_CP075881:c1144261-1143953 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTAATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT168418 WP_000948331.1 NZ_CP059180:c2011680-2011510 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT168418 NZ_CP075881:c1144500-1144264 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTTAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1