Detailed information of TA system TA168366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/YoeB-RelB
• Location: 1922061..1922590
• Replicon: chromosome
• Accession: NZ_CP059140
• Organism: Lactobacillus crispatus strain B4

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: -
• Locus tag: H0G71_RS09600
• Protein ID: WP_098046038.1
• Coordinates: 1922061..1922318 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: V5E284
• Locus tag: H0G71_RS09605
• Protein ID: WP_013087009.1
• Coordinates: 1922315..1922590 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0G71_RS09580	1917139..1917912	-	774	WP_005723724.1	tyrosine-protein phosphatase	-
H0G71_RS09585	1917968..1919467	-	1500	WP_023487729.1	glycerol kinase GlpK	-
H0G71_RS09590	1919592..1920407	+	816	WP_005720042.1	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-
H0G71_RS09595	1921126..1921959	-	834	WP_180808304.1	helix-turn-helix domain-containing protein	-
H0G71_RS09600	1922061..1922318	-	258	WP_098046038.1	Txe/YoeB family addiction module toxin	Toxin
H0G71_RS09605	1922315..1922590	-	276	WP_013087009.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H0G71_RS09610	1922784..1922915	-	132	WP_041811095.1	hypothetical protein	-
H0G71_RS09615	1923027..1923437	+	411	WP_131501200.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
H0G71_RS09620	1923458..1923736	+	279	WP_089147569.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H0G71_RS09625	1923806..1924492	+	687	WP_061205079.1	cobalamin biosynthesis protein CobQ	-
H0G71_RS09630	1924537..1925022	-	486	WP_013087013.1	C40 family peptidase	-
H0G71_RS09635	1925217..1925567	+	351	WP_005721217.1	hypothetical protein	-
H0G71_RS09640	1925965..1926246	+	282	WP_013087016.1	hypothetical protein	-
H0G71_RS09645	1926265..1927194	-	930	WP_089145949.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10159.60 Da
• Isoelectric Point: 8.6747

>T168366 WP_098046038.1 NZ_CP059140:c1922318-1922061 [Lactobacillus crispatus]
MIVAWTDDGWNDYVYWYDDGDCKKVSRINDLVKDMKRHPFTGIGKPEPLKRNLSGLWSRRIDSKNRIVYDCHKSMITIYS
CKDHY

Nucleotide

• Length: 258 bp

>T168366 NZ_CP075737:c666668-665346 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10597.82 Da
• Isoelectric Point: 4.5716

>AT168366 WP_013087009.1 NZ_CP059140:c1922590-1922315 [Lactobacillus crispatus]
MDAVNYSNFRKNLKHYFKQVNDDSEPLIVTNKEPEDNVVVLGKEDYDALVETLEIQSNQYLMDKIERGRKQVSEGTIKQH
ELIDPEQVTKS

Nucleotide

• Length: 276 bp

>AT168366 NZ_CP075737:c666934-666668 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: V5E284