Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4164245..4165079 | Replicon | chromosome |
| Accession | NZ_CP059130 | ||
| Organism | Escherichia coli strain Ec55 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A0H2VDB0 |
| Locus tag | H0X92_RS20360 | Protein ID | WP_000854688.1 |
| Coordinates | 4164702..4165079 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0H2VAY1 |
| Locus tag | H0X92_RS20355 | Protein ID | WP_001285596.1 |
| Coordinates | 4164245..4164625 (+) | Length | 127 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H0X92_RS20320 (4160530) | 4160530..4160985 | + | 456 | WP_000581502.1 | IrmA family protein | - |
| H0X92_RS20325 (4161064) | 4161064..4161297 | + | 234 | WP_001119727.1 | DUF905 family protein | - |
| H0X92_RS20330 (4161398) | 4161398..4162216 | + | 819 | WP_001234616.1 | DUF932 domain-containing protein | - |
| H0X92_RS20335 (4162271) | 4162271..4162756 | + | 486 | WP_000849564.1 | antirestriction protein | - |
| H0X92_RS20340 (4162772) | 4162772..4163248 | + | 477 | WP_001186726.1 | RadC family protein | - |
| H0X92_RS20345 (4163311) | 4163311..4163532 | + | 222 | WP_000692329.1 | DUF987 domain-containing protein | - |
| H0X92_RS20350 (4163551) | 4163551..4164195 | + | 645 | WP_000094917.1 | hypothetical protein | - |
| H0X92_RS20355 (4164245) | 4164245..4164625 | + | 381 | WP_001285596.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| H0X92_RS20360 (4164702) | 4164702..4165079 | + | 378 | WP_000854688.1 | TA system toxin CbtA family protein | Toxin |
| H0X92_RS20365 (4165076) | 4165076..4165564 | + | 489 | WP_000761701.1 | DUF5983 family protein | - |
| H0X92_RS20370 (4165581) | 4165581..4165778 | + | 198 | WP_000839260.1 | DUF957 domain-containing protein | - |
| H0X92_RS20375 (4165992) | 4165992..4167011 | + | 1020 | WP_000875213.1 | IS110 family transposase | - |
| H0X92_RS20380 (4167295) | 4167295..4168140 | + | 846 | WP_001280441.1 | DUF4942 domain-containing protein | - |
| H0X92_RS20385 (4168209) | 4168209..4168604 | + | 396 | WP_000208383.1 | DUF6088 family protein | - |
| H0X92_RS20390 (4168597) | 4168597..4169531 | + | 935 | Protein_4020 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT / kpsM / gspM | 4164245..4189839 | 25594 | |
| - | flank | IS/Tn | - | - | 4165992..4167011 | 1019 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14014.01 Da Isoelectric Point: 8.5221
>T168355 WP_000854688.1 NZ_CP059130:4164702-4165079 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR
Download Length: 378 bp
>T168355 NZ_CP075731:c4621919-4621608 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 127 a.a. Molecular weight: 13897.70 Da Isoelectric Point: 4.7959
>AT168355 WP_001285596.1 NZ_CP059130:4164245-4164625 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE
Download Length: 381 bp
>AT168355 NZ_CP075731:c4621607-4621317 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAAGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTACTG
CGAGCGATTGCCAATGATCCGCAACATGTACTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAAGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTACTG
CGAGCGATTGCCAATGATCCGCAACATGTACTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H2VDB0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H2VAY1 |