Detailed information of TA system TA168354

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4014961..4015615
• Replicon: chromosome
• Accession: NZ_CP059130
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: F4T2L4
• Locus tag: H0X92_RS19590
• Protein ID: WP_000244765.1
• Coordinates: 4014961..4015368 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: H0X92_RS19595
• Protein ID: WP_202745698.1
• Coordinates: 4015349..4015615 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS19570 (4010918)	4010918..4012651	-	1734	WP_000813195.1	single-stranded-DNA-specific exonuclease RecJ	-
H0X92_RS19575 (4012657)	4012657..4013367	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
H0X92_RS19580 (4013392)	4013392..4014288	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
H0X92_RS19585 (4014400)	4014400..4014921	+	522	WP_001055874.1	flavodoxin FldB	-
H0X92_RS19590 (4014961)	4014961..4015368	-	408	WP_000244765.1	protein YgfX	Toxin
H0X92_RS19595 (4015349)	4015349..4015615	-	267	WP_202745698.1	FAD assembly factor SdhE	Antitoxin
H0X92_RS19600 (4015858)	4015858..4016838	+	981	WP_000886084.1	tRNA-modifying protein YgfZ	-
H0X92_RS19605 (4016915)	4016915..4017574	-	660	WP_000250275.1	hemolysin III family protein	-
H0X92_RS19610 (4017738)	4017738..4018049	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
H0X92_RS19615 (4018094)	4018094..4019527	+	1434	WP_001296350.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16043.95 Da
• Isoelectric Point: 11.5202

>T168354 WP_000244765.1 NZ_CP059130:c4015368-4014961 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T168354 NZ_CP075731:c4343235-4343062 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10595.10 Da
• Isoelectric Point: 5.1601

>AT168354 WP_202745698.1 NZ_CP059130:c4015615-4015349 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLEYDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT168354 NZ_CP075731:c4343559-4343263 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A454A7D7