Detailed information of TA system TA168352

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2993912..2994743
• Replicon: chromosome
• Accession: NZ_CP059130
• Organism: Escherichia coli strain Ec55

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: H0X92_RS14825
• Protein ID: WP_000854815.1
• Coordinates: 2994369..2994743 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A061Y7A8
• Locus tag: H0X92_RS14820
• Protein ID: WP_001280918.1
• Coordinates: 2993912..2994280 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X92_RS14775 (2989001)	2989001..2989747	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H0X92_RS14780 (2989830)	2989830..2990180	+	351	Protein_2915	hypothetical protein	-
H0X92_RS14785 (2990196)	2990196..2990606	+	411	WP_000846703.1	hypothetical protein	-
H0X92_RS14790 (2990827)	2990827..2991645	+	819	WP_001542275.1	DUF932 domain-containing protein	-
H0X92_RS14795 (2991645)	2991645..2991890	+	246	WP_001164966.1	hypothetical protein	-
H0X92_RS14800 (2991984)	2991984..2992457	+	474	WP_001542276.1	antirestriction protein	-
H0X92_RS14805 (2992473)	2992473..2992949	+	477	WP_001186200.1	RadC family protein	-
H0X92_RS14810 (2993012)	2993012..2993233	+	222	WP_000692345.1	DUF987 domain-containing protein	-
H0X92_RS14815 (2993252)	2993252..2993896	+	645	WP_000086752.1	hypothetical protein	-
H0X92_RS14820 (2993912)	2993912..2994280	+	369	WP_001280918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H0X92_RS14825 (2994369)	2994369..2994743	+	375	WP_000854815.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
H0X92_RS14830 (2994740)	2994740..2994934	+	195	WP_000988600.1	DUF5983 family protein	-
H0X92_RS14835 (2994980)	2994980..2995060	+	81	Protein_2926	hypothetical protein	-
H0X92_RS26640 (2995349)	2995349..2995429	-	81	WP_023441679.1	hypothetical protein	-
H0X92_RS26645 (2995408)	2995408..2995731	+	324	WP_225469267.1	EutP/PduV family microcompartment system protein	-
H0X92_RS14850 (2995832)	2995832..2996161	-	330	WP_000450409.1	DUF496 family protein	-
H0X92_RS14855 (2996333)	2996333..2997391	-	1059	WP_001200889.1	FUSC family protein	-
H0X92_RS14860 (2997589)	2997589..2998062	-	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
H0X92_RS14865 (2998181)	2998181..2999347	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T168352 WP_000854815.1 NZ_CP059130:2994369-2994743 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T168352 NZ_CP075731:4083615-4083767 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13811.70 Da
• Isoelectric Point: 6.4767

>AT168352 WP_001280918.1 NZ_CP059130:2993912-2994280 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT168352 NZ_CP075731:c4083566-4083509 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: A0A066T988

Antitoxin

• AlphaFold DB: A0A061Y7A8