Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1263917..1264535 Replicon chromosome
Accession NZ_CP059130
Organism Escherichia coli strain Ec55

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H0X92_RS05995 Protein ID WP_001291435.1
Coordinates 1263917..1264135 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H0X92_RS06000 Protein ID WP_000344800.1
Coordinates 1264161..1264535 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X92_RS05960 (1259204) 1259204..1259776 + 573 WP_000779831.1 YbaY family lipoprotein -
H0X92_RS05965 (1259807) 1259807..1260118 - 312 WP_000409908.1 MGMT family protein -
H0X92_RS05975 (1260497) 1260497..1260850 + 354 WP_000878135.1 DUF1428 family protein -
H0X92_RS05980 (1260892) 1260892..1262442 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H0X92_RS05985 (1262606) 1262606..1263076 - 471 WP_000136192.1 YlaC family protein -
H0X92_RS05990 (1263192) 1263192..1263743 - 552 WP_000102539.1 maltose O-acetyltransferase -
H0X92_RS05995 (1263917) 1263917..1264135 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H0X92_RS06000 (1264161) 1264161..1264535 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H0X92_RS06005 (1265081) 1265081..1268230 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
H0X92_RS06010 (1268253) 1268253..1269446 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168337 WP_001291435.1 NZ_CP059130:c1264135-1263917 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T168337 NZ_CP075724:c58268-57987 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT168337 WP_000344800.1 NZ_CP059130:c1264535-1264161 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT168337 NZ_CP075724:c58509-58258 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References